Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ferulic acid | strong activation of FaeD and FaeT resulting in 15fold and 4fold increase of the FaeD and FaeT activities, respectively, at 0.5 mM | Dickeya dadantii |
Cloned (Comment) | Organism |
---|---|
genes faeT and faeD, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3) | Dickeya dadantii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | feruloyl esterase FaeT | Dickeya dadantii | 5737 | - |
extracellular | feruloyl esterase FaeD, which is an extracellular protein secreted by the Out system | Dickeya dadantii | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no difference in enzymatic activity through addition of cations Ca2+, Cu2+, Fe2+, Mg2+, or Mn2+ at 0.1-1 mM or 10 mM EDTA | Dickeya dadantii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dickeya dadantii | - |
formerly Erwinia chrysanthemi, genes faeT and faeD | - |
Dickeya dadantii 3937 | - |
formerly Erwinia chrysanthemi, genes faeT and faeD | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.005 | - |
about, recombinant feruoyl esterase FaeD, pH 7.5, 37°C, substrate 4-nitrophenyl acetate | Dickeya dadantii |
0.0083 | - |
about, recombinant feruoyl esterase FaeT, pH 7.5, 37°C, substrate 4-nitrophenyl acetate | Dickeya dadantii |
0.0267 | - |
about, recombinant feruoyl esterase FaeD, pH 7.5, 37°C, substrate 4-nitrophenyl acetate, in presence of 0.5 mM ferulic acid | Dickeya dadantii |
0.033 | - |
about, recombinant feruoyl esterase FaeT, pH 7.5, 37°C, substrate 4-nitrophenyl acetate, in presence of 0.5 mM ferulic acid | Dickeya dadantii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Dickeya dadantii | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Dickeya dadantii 3937 | 4-nitrophenol + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FaeD | - |
Dickeya dadantii |
FaeT | - |
Dickeya dadantii |
More | FaeD and FaeT belong to the carbohydrate esterase family CE10 | Dickeya dadantii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Dickeya dadantii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Dickeya dadantii |
Organism | Comment | Expression |
---|---|---|
Dickeya dadantii | expression of genes faeT and faeD is not significantly affected by the addition of acetosyringone, benzoic acid, cinnamic acid, p-coumaric acid, vanillic acid, arabinogalactan, arabinose, galactose, galacturonate, mannose, pectin, polygalacturonate, rhamnose, salicine, xylose, or xylan | additional information |
Dickeya dadantii | feruoyl esterases FaeD and FaeT are induced by ferulic acid. The product of the adjacent gene faeR is involved in the positive control of faeD in response to ferulic acid. Moreover, ferulic acid acts in synergy with polygalacturonate to induce pectate lyases, the main virulence determinant of soft rot disease | up |
General Information | Comment | Organism |
---|---|---|
malfunction | when gene faeT is inactivated, the size of the esterase halo clearly decreased. When gene faeD is inactivated, only very low residual feruoyl esterase activity is detected, as observed in the faeD faeT double mutant | Dickeya dadantii |
physiological function | the two feruloyl esterases, FaeD and FaeT, cleave the ester link between ferulate and the pectic or xylan chains. FaeD is an extracellular protein secreted by the Out system, responsible for pectinase secretion. Feruloyl esterases dissociate internal cross-links in the polysaccharide network of the plant cell wall, suppress the polysaccharide esterifications, and liberate ferulic acid, which contributes to the induction of pectate lyases. Together, these effects of feruloyl esterases could facilitate soft rot disease caused by pectinolytic bacteria. FaeD appears to be responsible for most feruloyl esterase activity in Dickeya dadantii | Dickeya dadantii |