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Search term: 3T3-L1 cell

Results 1 - 74 of 74
EC Number
Source Tissue
Commentary
Reference
11beta-hydroxysteroid dehydrogenase
-
11beta-hydroxysteroid dehydrogenase
differentiation of cells causes a strong increase in 11beta-hydroxysteroid dehydrogenase protein levels, occuring late in the differentiation protocol. Reduction of 11beta-hydroxysteroid dehydrogenase activity in 3T3-L1 fibroblasts, achieved by pharmacological inhibition or adenovirally mediated delivery of short hairpin RNA constructs, specifically blocks the ability of inactive glucocorticoids to drive 3T3-L1 differentiation. Even modest increases in exogenous 11beta-hydroxysteroid dehydrogenase expression in 3T3-L1 fibroblasts, to levels comparable with endogenous 1 11beta-hydroxysteroid dehydrogenase in differentiated 3T3-L1 adipocytes, are sufficient to block adipogenesis
prostaglandin-F synthase
AKR1B3 mRNA 9 is expressed in preadipocytes, and its level increases about 4fold at day 1 after initiation of adipocyte differentiation, and then quickly decreases the following day to a level lower than that in the preadipocytes
IMP dehydrogenase
-
malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)
-
phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)
-
glycerol-3-phosphate dehydrogenase (NAD+)
-
4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase
-
stearoyl-CoA 9-desaturase
-
stearoyl-CoA 9-desaturase
conjugated linoleic acid decreases SCD1 gene expression
xanthine dehydrogenase
mRNA expression increases 8-fold during the first 12 hr postinduction and subsequently declines to preinduction levels by day 3. Xanthine dehydrogenase activity is maximally induced at 24 hr postinduction, while xanthine oxidase activity remains relatively unchanged
aldehyde oxidase
preadipocytes have a very low expression of AOX1, in 2 days differentiated cells AOX1 is induced and is not further upregulated in 3, 7 and 9 days differentiated cells. AOX1 mRNA is nearly four-fold higher in 2 days differentiated cells when compared to preadipocytes and doubles from days 2 to 6
13,14-dehydro-15-oxoprostaglandin 13-reductase
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acyl-CoA oxidase
-
all-trans-retinol 13,14-reductase
-
NAD(P)H oxidase (H2O2-forming)
-
nicotinamide N-methyltransferase
elevated enzyme activity during cell differentiation in adipose tissue
phosphatidylethanolamine N-methyltransferase
-
alpha-tubulin N-acetyltransferase
-
glycerol-3-phosphate 1-O-acyltransferase
Gpat3 mRNA increases 60fold during differentiation of 3T3-L1 preadipocytes to mature adipocytes, suggesting a critical role in adipocytes
glycerol-3-phosphate 1-O-acyltransferase
pre- and post-adipocytes
diacylglycerol O-acyltransferase
3T3-L1 fibroblasts differentiated into adipocytes
carnitine O-palmitoyltransferase
CPT I and CPT II
histone acetyltransferase
-
fatty-acid synthase
-
ATP citrate synthase
-
glycogen(starch) synthase
synthetic peroxisome proliferator-activated receptor beta/delta, and gamma agonists markedly up-regulate Gys-2 mRNA and protein expression in mouse 3T3-L1 adipocytes
protein O-GlcNAc transferase
-
ceramide glucosyltransferase
-
nicotinamide phosphoribosyltransferase
-
phosphatidylinositol 3-kinase
-
1-phosphatidylinositol-3-phosphate 5-kinase
-
phosphatidylinositol-4-phosphate 3-kinase
adipocyte
1-phosphatidylinositol 4-kinase
-
receptor protein-tyrosine kinase
-
receptor protein-tyrosine kinase
HER2 is absent in undifferentiated cultures of adipocytes, not subjected to hormonal treatment but processed at the same time as the differentiating cultures from day 0 to day 14. Expression of HER2 significantly augments during the differentiation of preadipocytes. HER2 acculmulation reaches a maximum at day 9
non-specific serine/threonine protein kinase
isozyme PKNalpha
cAMP-dependent protein kinase
-
[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase
-
inositol-hexakisphosphate kinase
-
adenylate kinase
-
phosphatidate cytidylyltransferase
;
estrone sulfotransferase
-
triacylglycerol lipase
-
triacylglycerol lipase
adipocytes, enzyme expression during differentiation of adipocytes, maximal at day 8, overview
lipoprotein lipase
preadipocyte cell line
lipoprotein lipase
scopoletin significantly increases lipoprotein lipase activity in 3T3-L1 adipocytes
lysophospholipase
-
hormone-sensitive lipase
-
alkaline phosphatase
activity increases during adipogenesis
phosphatidate phosphatase
-
phosphatidate phosphatase
lipin-1 and lipin-2
fructose-2,6-bisphosphate 2-phosphatase
adipocyte cell line
protein-tyrosine-phosphatase
-
phosphoinositide phospholipase C
-
cystinyl aminopeptidase
-
tripeptide aminopeptidase
-
proprotein convertase 5
-
cathepsin K
-
cathepsin K
ATCC CL-173
matrix metalloproteinase-11
-
GTP cyclohydrolase I
adipocyte cell line
small monomeric GTPase
-
sulfinoalanine decarboxylase
-
phosphatidylserine decarboxylase
-
sphinganine-1-phosphate aldolase
-
sphinganine-1-phosphate aldolase
CL 173 (ATCC)
Peptidylprolyl isomerase
-
acetate-CoA ligase
marked induction of AceCS1 mRNA and protein during differentiation of 3T3-L1 cells, neither AceCS2 mRNA nor protein is detected in undifferentiated or differentiated 3T3-L1 cells
acetoacetate-CoA ligase
-
long-chain-fatty-acid-CoA ligase
-
CTP synthase (glutamine hydrolysing)
-
pyruvate carboxylase
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Results 1 - 74 of 74