EC Number |
Recommended Name |
Source Tissue |
Reference |
---|
4.1.2.27 | sphinganine-1-phosphate aldolase |
3T3-L1 cell |
CL 173 (ATCC) |
5119 |
2.3.1.20 | diacylglycerol O-acyltransferase |
3T3-L1 cell |
3T3-L1 fibroblasts differentiated into adipocytes |
486513 |
2.3.1.21 | carnitine O-palmitoyltransferase |
3T3-L1 cell |
CPT I and CPT II |
486577 |
2.7.1.154 | phosphatidylinositol-4-phosphate 3-kinase |
3T3-L1 cell |
adipocyte |
640921 |
6.2.1.1 | acetate-CoA ligase |
3T3-L1 cell |
marked induction of AceCS1 mRNA and protein during differentiation of 3T3-L1 cells, neither AceCS2 mRNA nor protein is detected in undifferentiated or differentiated 3T3-L1 cells |
652101 |
2.3.1.85 | fatty-acid synthase system |
3T3-L1 cell |
- |
660895 |
2.7.11.1 | non-specific serine/threonine protein kinase |
3T3-L1 cell |
isozyme PKNalpha |
662053 |
6.2.1.3 | long-chain-fatty-acid-CoA ligase |
3T3-L1 cell |
- |
662593 |
3.1.3.46 | fructose-2,6-bisphosphate 2-phosphatase |
3T3-L1 cell |
adipocyte cell line |
664718 |
3.1.1.3 | triacylglycerol lipase |
3T3-L1 cell |
adipocytes, enzyme expression during differentiation of adipocytes, maximal at day 8, overview |
664719 |
2.7.11.31 | [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase |
3T3-L1 cell |
- |
665636, 702510, 703277 |
3.5.4.16 | GTP cyclohydrolase I |
3T3-L1 cell |
adipocyte cell line |
667769 |
3.4.11.3 | cystinyl aminopeptidase |
3T3-L1 cell |
- |
-, 669191, 683133, 709666, 754109, 755404 |
1.3.3.6 | acyl-CoA oxidase |
3T3-L1 cell |
- |
671758 |
1.17.1.4 | xanthine dehydrogenase |
3T3-L1 cell |
mRNA expression increases 8-fold during the first 12 hr postinduction and subsequently declines to preinduction levels by day 3. Xanthine dehydrogenase activity is maximally induced at 24 hr postinduction, while xanthine oxidase activity remains relatively unchanged |
672979 |
1.14.19.1 | stearoyl-CoA 9-desaturase |
3T3-L1 cell |
conjugated linoleic acid decreases SCD1 gene expression |
675626 |
3.4.21.B26 | proprotein convertase 5 |
3T3-L1 cell |
- |
675967 |
2.7.1.67 | 1-phosphatidylinositol 4-kinase |
3T3-L1 cell |
- |
676001 |
3.1.3.1 | alkaline phosphatase |
3T3-L1 cell |
activity increases during adipogenesis |
677519 |
2.4.1.80 | ceramide glucosyltransferase |
3T3-L1 cell |
- |
679436 |
3.4.11.4 | tripeptide aminopeptidase |
3T3-L1 cell |
- |
679509 |
3.1.1.34 | lipoprotein lipase |
3T3-L1 cell |
scopoletin significantly increases lipoprotein lipase activity in 3T3-L1 adipocytes |
680239 |
4.1.2.27 | sphinganine-1-phosphate aldolase |
3T3-L1 cell |
- |
681269 |
1.6.3.1 | NAD(P)H oxidase (H2O2-forming) |
3T3-L1 cell |
- |
685345 |
1.1.1.44 | phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating) |
3T3-L1 cell |
- |
685541 |
2.4.1.11 | glycogen(starch) synthase |
3T3-L1 cell |
synthetic peroxisome proliferator-activated receptor beta/delta, and gamma agonists markedly up-regulate Gys-2 mRNA and protein expression in mouse 3T3-L1 adipocytes |
686019 |
1.1.1.146 | 11beta-hydroxysteroid dehydrogenase |
3T3-L1 cell |
- |
686411, 697625, 700121, 700180, 712594, 740806 |
1.3.1.48 | 13,14-dehydro-15-oxoprostaglandin 13-reductase |
3T3-L1 cell |
- |
687562 |
1.14.19.1 | stearoyl-CoA 9-desaturase |
3T3-L1 cell |
- |
687766, 746212 |
2.3.1.15 | glycerol-3-phosphate 1-O-acyltransferase |
3T3-L1 cell |
pre- and post-adipocytes |
688948 |
2.3.1.48 | histone acetyltransferase |
3T3-L1 cell |
- |
688978 |
6.4.1.1 | pyruvate carboxylase |
3T3-L1 cell |
- |
690828, 705517 |
6.2.1.16 | acetoacetate-CoA ligase |
3T3-L1 cell |
- |
691065, 727116 |
1.1.1.146 | 11beta-hydroxysteroid dehydrogenase |
3T3-L1 cell |
differentiation of cells causes a strong increase in 11beta-hydroxysteroid dehydrogenase protein levels, occuring late in the differentiation protocol. Reduction of 11beta-hydroxysteroid dehydrogenase activity in 3T3-L1 fibroblasts, achieved by pharmacological inhibition or adenovirally mediated delivery of short hairpin RNA constructs, specifically blocks the ability of inactive glucocorticoids to drive 3T3-L1 differentiation. Even modest increases in exogenous 11beta-hydroxysteroid dehydrogenase expression in 3T3-L1 fibroblasts, to levels comparable with endogenous 1 11beta-hydroxysteroid dehydrogenase in differentiated 3T3-L1 adipocytes, are sufficient to block adipogenesis |
692987 |
2.4.1.255 | protein O-GlcNAc transferase |
3T3-L1 cell |
- |
693100 |
3.1.1.5 | lysophospholipase |
3T3-L1 cell |
- |
693213 |
2.7.10.1 | receptor protein-tyrosine kinase |
3T3-L1 cell |
HER2 is absent in undifferentiated cultures of adipocytes, not subjected to hormonal treatment but processed at the same time as the differentiating cultures from day 0 to day 14. Expression of HER2 significantly augments during the differentiation of preadipocytes. HER2 acculmulation reaches a maximum at day 9 |
693316 |
4.1.1.29 | sulfinoalanine decarboxylase |
3T3-L1 cell |
- |
693792 |
2.7.11.11 | cAMP-dependent protein kinase |
3T3-L1 cell |
- |
694155 |
3.4.24.B3 | matrix metalloproteinase-11 |
3T3-L1 cell |
- |
694496 |
3.4.22.38 | cathepsin K |
3T3-L1 cell |
- |
695943 |
3.4.22.38 | cathepsin K |
3T3-L1 cell |
ATCC CL-173 |
697619 |
1.2.3.1 | aldehyde oxidase |
3T3-L1 cell |
preadipocytes have a very low expression of AOX1, in 2 days differentiated cells AOX1 is induced and is not further upregulated in 3, 7 and 9 days differentiated cells. AOX1 mRNA is nearly four-fold higher in 2 days differentiated cells when compared to preadipocytes and doubles from days 2 to 6 |
697933 |
1.1.1.8 | glycerol-3-phosphate dehydrogenase (NAD+) |
3T3-L1 cell |
- |
699948, 760740 |
3.6.5.2 | small monomeric GTPase |
3T3-L1 cell |
- |
699992, 701352 |
1.3.99.23 | all-trans-retinol 13,14-reductase |
3T3-L1 cell |
- |
-, 700994, 763507 |
2.3.1.15 | glycerol-3-phosphate 1-O-acyltransferase |
3T3-L1 cell |
Gpat3 mRNA increases 60fold during differentiation of 3T3-L1 preadipocytes to mature adipocytes, suggesting a critical role in adipocytes |
701623 |
2.1.1.1 | nicotinamide N-methyltransferase |
3T3-L1 cell |
elevated enzyme activity during cell differentiation in adipose tissue |
701990 |
1.1.1.205 | IMP dehydrogenase |
3T3-L1 cell |
- |
702061 |
3.4.22.52 | calpain-1 |
3T3-L1 cell |
- |
707364 |
3.1.1.3 | triacylglycerol lipase |
3T3-L1 cell |
- |
707547, 708289 |
3.1.3.4 | phosphatidate phosphatase |
3T3-L1 cell |
lipin-1 and lipin-2 |
707555 |
3.1.3.4 | phosphatidate phosphatase |
3T3-L1 cell |
- |
680814, 708998, 709417, 709945, 714316 |
3.1.1.34 | lipoprotein lipase |
3T3-L1 cell |
preadipocyte cell line |
-, 709009 |
2.7.1.137 | phosphatidylinositol 3-kinase |
3T3-L1 cell |
- |
709772 |
1.1.1.188 | prostaglandin-F synthase |
3T3-L1 cell |
AKR1B3 mRNA 9 is expressed in preadipocytes, and its level increases about 4fold at day 1 after initiation of adipocyte differentiation, and then quickly decreases the following day to a level lower than that in the preadipocytes |
712494 |
3.1.1.79 | hormone-sensitive lipase |
3T3-L1 cell |
- |
714794 |
4.1.1.65 | phosphatidylserine decarboxylase |
3T3-L1 cell |
- |
715603 |
5.2.1.8 | peptidylprolyl isomerase |
3T3-L1 cell |
- |
715609 |
3.1.4.11 | phosphoinositide phospholipase C |
3T3-L1 cell |
- |
716113 |
2.1.1.17 | phosphatidylethanolamine N-methyltransferase |
3T3-L1 cell |
- |
719858 |
2.7.10.1 | receptor protein-tyrosine kinase |
3T3-L1 cell |
- |
721490 |
2.7.4.3 | adenylate kinase |
3T3-L1 cell |
- |
722732 |
2.7.1.150 | 1-phosphatidylinositol-3-phosphate 5-kinase |
3T3-L1 cell |
- |
723139 |
2.8.2.4 | estrone sulfotransferase |
3T3-L1 cell |
- |
-, 725979, 760988 |
6.3.4.2 | CTP synthase (glutamine hydrolysing) |
3T3-L1 cell |
- |
727427 |
3.1.3.48 | protein-tyrosine-phosphatase |
3T3-L1 cell |
- |
729290 |
1.14.18.6 | 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase |
3T3-L1 cell |
- |
734433 |
2.3.1.108 | alpha-tubulin N-acetyltransferase |
3T3-L1 cell |
- |
735631 |
2.3.3.8 | ATP citrate synthase |
3T3-L1 cell |
- |
736797 |
2.7.4.21 | inositol-hexakisphosphate 5-kinase |
3T3-L1 cell |
- |
738392 |
2.7.7.41 | phosphatidate cytidylyltransferase |
3T3-L1 cell |
- |
738826 |
1.1.1.40 | malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) |
3T3-L1 cell |
- |
739138 |
2.7.11.24 | mitogen-activated protein kinase |
3T3-L1 cell |
- |
740424 |
1.11.1.20 | prostamide/prostaglandin F2alpha synthase |
3T3-L1 cell |
- |
740711 |
1.14.11.4 | procollagen-lysine 5-dioxygenase |
3T3-L1 cell |
- |
745769 |
1.14.11.66 | [histone H3]-trimethyl-L-lysine9 demethylase |
3T3-L1 cell |
- |
746217 |
1.14.19.1 | stearoyl-CoA 9-desaturase |
3T3-L1 cell |
isozyme SCD1 is dramatically induced during 3T3L1 differentiation |
746542 |
5.6.2.2 | DNA topoisomerase (ATP-hydrolysing) |
3T3-L1 cell |
- |
747492 |
3.1.3.86 | phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase |
3T3-L1 cell |
- |
750315 |
3.1.3.46 | fructose-2,6-bisphosphate 2-phosphatase |
3T3-L1 cell |
- |
751121 |
3.4.21.46 | complement factor D |
3T3-L1 cell |
- |
755129 |
2.1.1.348 | mRNA m6A methyltransferase |
3T3-L1 cell |
- |
757661 |
2.3.1.15 | glycerol-3-phosphate 1-O-acyltransferase |
3T3-L1 cell |
- |
756106, 757868 |
2.3.1.20 | diacylglycerol O-acyltransferase |
3T3-L1 cell |
- |
758179 |
2.4.3.1 | beta-galactoside alpha-(2,6)-sialyltransferase |
3T3-L1 cell |
- |
759485 |
2.4.2.12 | nicotinamide phosphoribosyltransferase |
3T3-L1 cell |
- |
735872, 759503 |
2.7.11.1 | non-specific serine/threonine protein kinase |
3T3-L1 cell |
- |
761503 |