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Literature summary for 4.1.2.27 extracted from
- A rapid fluorescence assay for sphingosine-1-phosphate lyase enzyme activity (2007), J. Lipid Res., 48, 2769-2778.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| FTY720 | inhibits at 0.03 mM | Homo sapiens |  |
| Semicarbazide | nonspecific SPL inhibitor, reduces SPL activity in vitro by about 70% at 2 mM | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0146 | - |
sphingosine 1-phosphate | in 0.6 mM EDTA, 0.4 mM pyridoxal 5'-phosphate, 3 mM dithiothreitol mM sucrose, 36 mM potassium phosphate buffer, and 36 mM NaF containing 0.08% Triton X-100 | Homo sapiens | |
| 0.0201 | - |
Dihydrosphingosine 1-phosphonate | in 0.6 mM EDTA, 0.4 mM pyridoxal 5'-phosphate, 3 mM dithiothreitol mM sucrose, 36 mM potassium phosphate buffer, and 36 mM NaF containing 0.08% Triton X-100 | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| 3T3-L1 cell | - |
Mus musculus | - |
| DLD-1 cell | - |
Homo sapiens | - |
| HEK-293 cell | - |
Homo sapiens | - |
| liver | - |
Mus musculus | - |
| thymus | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Dihydrosphingosine 1-phosphonate | - |
Mus musculus | Aminoethylphosphonate + palmidaldehyde | - |
? | |
| Dihydrosphingosine 1-phosphonate | - |
Homo sapiens | Aminoethylphosphonate + palmidaldehyde | - |
? | |
| phytosphingosine-1-phosphate | - |
Mus musculus | ? | - |
? | |
| phytosphingosine-1-phosphate | - |
Homo sapiens | ? | - |
? | |
| sphingosine 1-phosphate | - |
Mus musculus | phosphoethanolamine + palmitaldehyde | - |
? | |
| sphingosine 1-phosphate | - |
Homo sapiens | phosphoethanolamine + palmitaldehyde | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| S1P lyase | - |
Mus musculus |
| S1P lyase | - |
Homo sapiens |
| sphingosine-1-phosphate lyase | - |
Mus musculus |
| sphingosine-1-phosphate lyase | - |
Homo sapiens |
| SPL | - |
Mus musculus |
| SPL | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Mus musculus | |
| pyridoxal 5'-phosphate | - |
Homo sapiens | |
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