EC Number |
Recommended Name |
Source Tissue |
Reference |
---|
2.7.1.67 | 1-phosphatidylinositol 4-kinase |
3T3-L1 cell |
- |
676001 |
2.7.1.150 | 1-phosphatidylinositol-3-phosphate 5-kinase |
3T3-L1 cell |
- |
723139 |
1.1.1.146 | 11beta-hydroxysteroid dehydrogenase |
3T3-L1 cell |
- |
686411, 697625, 700121, 700180, 712594, 740806 |
1.1.1.146 | 11beta-hydroxysteroid dehydrogenase |
3T3-L1 cell |
differentiation of cells causes a strong increase in 11beta-hydroxysteroid dehydrogenase protein levels, occuring late in the differentiation protocol. Reduction of 11beta-hydroxysteroid dehydrogenase activity in 3T3-L1 fibroblasts, achieved by pharmacological inhibition or adenovirally mediated delivery of short hairpin RNA constructs, specifically blocks the ability of inactive glucocorticoids to drive 3T3-L1 differentiation. Even modest increases in exogenous 11beta-hydroxysteroid dehydrogenase expression in 3T3-L1 fibroblasts, to levels comparable with endogenous 1 11beta-hydroxysteroid dehydrogenase in differentiated 3T3-L1 adipocytes, are sufficient to block adipogenesis |
692987 |
1.3.1.48 | 13,14-dehydro-15-oxoprostaglandin 13-reductase |
3T3-L1 cell |
- |
687562 |
1.14.18.6 | 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase |
3T3-L1 cell |
- |
734433 |
6.2.1.1 | acetate-CoA ligase |
3T3-L1 cell |
marked induction of AceCS1 mRNA and protein during differentiation of 3T3-L1 cells, neither AceCS2 mRNA nor protein is detected in undifferentiated or differentiated 3T3-L1 cells |
652101 |
6.2.1.16 | acetoacetate-CoA ligase |
3T3-L1 cell |
- |
691065, 727116 |
1.3.3.6 | acyl-CoA oxidase |
3T3-L1 cell |
- |
671758 |
2.7.4.3 | adenylate kinase |
3T3-L1 cell |
- |
722732 |
1.2.3.1 | aldehyde oxidase |
3T3-L1 cell |
preadipocytes have a very low expression of AOX1, in 2 days differentiated cells AOX1 is induced and is not further upregulated in 3, 7 and 9 days differentiated cells. AOX1 mRNA is nearly four-fold higher in 2 days differentiated cells when compared to preadipocytes and doubles from days 2 to 6 |
697933 |
3.1.3.1 | alkaline phosphatase |
3T3-L1 cell |
activity increases during adipogenesis |
677519 |
1.3.99.23 | all-trans-retinol 13,14-reductase |
3T3-L1 cell |
- |
-, 700994, 763507 |
2.3.1.108 | alpha-tubulin N-acetyltransferase |
3T3-L1 cell |
- |
735631 |
2.3.3.8 | ATP citrate synthase |
3T3-L1 cell |
- |
736797 |
2.4.3.1 | beta-galactoside alpha-(2,6)-sialyltransferase |
3T3-L1 cell |
- |
759485 |
3.4.22.52 | calpain-1 |
3T3-L1 cell |
- |
707364 |
2.7.11.11 | cAMP-dependent protein kinase |
3T3-L1 cell |
- |
694155 |
2.3.1.21 | carnitine O-palmitoyltransferase |
3T3-L1 cell |
CPT I and CPT II |
486577 |
3.4.22.38 | cathepsin K |
3T3-L1 cell |
- |
695943 |
3.4.22.38 | cathepsin K |
3T3-L1 cell |
ATCC CL-173 |
697619 |
2.4.1.80 | ceramide glucosyltransferase |
3T3-L1 cell |
- |
679436 |
3.4.21.46 | complement factor D |
3T3-L1 cell |
- |
755129 |
6.3.4.2 | CTP synthase (glutamine hydrolysing) |
3T3-L1 cell |
- |
727427 |
3.4.11.3 | cystinyl aminopeptidase |
3T3-L1 cell |
- |
-, 669191, 683133, 709666, 754109, 755404 |
2.3.1.20 | diacylglycerol O-acyltransferase |
3T3-L1 cell |
- |
758179 |
2.3.1.20 | diacylglycerol O-acyltransferase |
3T3-L1 cell |
3T3-L1 fibroblasts differentiated into adipocytes |
486513 |
5.6.2.2 | DNA topoisomerase (ATP-hydrolysing) |
3T3-L1 cell |
- |
747492 |
2.8.2.4 | estrone sulfotransferase |
3T3-L1 cell |
- |
-, 725979, 760988 |
2.3.1.85 | fatty-acid synthase system |
3T3-L1 cell |
- |
660895 |
3.1.3.46 | fructose-2,6-bisphosphate 2-phosphatase |
3T3-L1 cell |
- |
751121 |
3.1.3.46 | fructose-2,6-bisphosphate 2-phosphatase |
3T3-L1 cell |
adipocyte cell line |
664718 |
2.3.1.15 | glycerol-3-phosphate 1-O-acyltransferase |
3T3-L1 cell |
- |
756106, 757868 |
2.3.1.15 | glycerol-3-phosphate 1-O-acyltransferase |
3T3-L1 cell |
Gpat3 mRNA increases 60fold during differentiation of 3T3-L1 preadipocytes to mature adipocytes, suggesting a critical role in adipocytes |
701623 |
2.3.1.15 | glycerol-3-phosphate 1-O-acyltransferase |
3T3-L1 cell |
pre- and post-adipocytes |
688948 |
1.1.1.8 | glycerol-3-phosphate dehydrogenase (NAD+) |
3T3-L1 cell |
- |
699948, 760740 |
2.4.1.11 | glycogen(starch) synthase |
3T3-L1 cell |
synthetic peroxisome proliferator-activated receptor beta/delta, and gamma agonists markedly up-regulate Gys-2 mRNA and protein expression in mouse 3T3-L1 adipocytes |
686019 |
3.5.4.16 | GTP cyclohydrolase I |
3T3-L1 cell |
adipocyte cell line |
667769 |
2.3.1.48 | histone acetyltransferase |
3T3-L1 cell |
- |
688978 |
3.1.1.79 | hormone-sensitive lipase |
3T3-L1 cell |
- |
714794 |
1.1.1.205 | IMP dehydrogenase |
3T3-L1 cell |
- |
702061 |
2.7.4.21 | inositol-hexakisphosphate 5-kinase |
3T3-L1 cell |
- |
738392 |
3.1.1.34 | lipoprotein lipase |
3T3-L1 cell |
preadipocyte cell line |
-, 709009 |
3.1.1.34 | lipoprotein lipase |
3T3-L1 cell |
scopoletin significantly increases lipoprotein lipase activity in 3T3-L1 adipocytes |
680239 |
6.2.1.3 | long-chain-fatty-acid-CoA ligase |
3T3-L1 cell |
- |
662593 |
3.1.1.5 | lysophospholipase |
3T3-L1 cell |
- |
693213 |
1.1.1.40 | malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) |
3T3-L1 cell |
- |
739138 |
3.4.24.B3 | matrix metalloproteinase-11 |
3T3-L1 cell |
- |
694496 |
2.7.11.24 | mitogen-activated protein kinase |
3T3-L1 cell |
- |
740424 |
2.1.1.348 | mRNA m6A methyltransferase |
3T3-L1 cell |
- |
757661 |
1.6.3.1 | NAD(P)H oxidase (H2O2-forming) |
3T3-L1 cell |
- |
685345 |
2.1.1.1 | nicotinamide N-methyltransferase |
3T3-L1 cell |
elevated enzyme activity during cell differentiation in adipose tissue |
701990 |
2.4.2.12 | nicotinamide phosphoribosyltransferase |
3T3-L1 cell |
- |
735872, 759503 |
2.7.11.1 | non-specific serine/threonine protein kinase |
3T3-L1 cell |
- |
761503 |
2.7.11.1 | non-specific serine/threonine protein kinase |
3T3-L1 cell |
isozyme PKNalpha |
662053 |
5.2.1.8 | peptidylprolyl isomerase |
3T3-L1 cell |
- |
715609 |
2.7.7.41 | phosphatidate cytidylyltransferase |
3T3-L1 cell |
- |
738826 |
3.1.3.4 | phosphatidate phosphatase |
3T3-L1 cell |
- |
680814, 708998, 709417, 709945, 714316 |
3.1.3.4 | phosphatidate phosphatase |
3T3-L1 cell |
lipin-1 and lipin-2 |
707555 |
2.1.1.17 | phosphatidylethanolamine N-methyltransferase |
3T3-L1 cell |
- |
719858 |
2.7.1.137 | phosphatidylinositol 3-kinase |
3T3-L1 cell |
- |
709772 |
3.1.3.86 | phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase |
3T3-L1 cell |
- |
750315 |
2.7.1.154 | phosphatidylinositol-4-phosphate 3-kinase |
3T3-L1 cell |
adipocyte |
640921 |
4.1.1.65 | phosphatidylserine decarboxylase |
3T3-L1 cell |
- |
715603 |
1.1.1.44 | phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating) |
3T3-L1 cell |
- |
685541 |
3.1.4.11 | phosphoinositide phospholipase C |
3T3-L1 cell |
- |
716113 |
1.14.11.4 | procollagen-lysine 5-dioxygenase |
3T3-L1 cell |
- |
745769 |
3.4.21.B26 | proprotein convertase 5 |
3T3-L1 cell |
- |
675967 |
1.1.1.188 | prostaglandin-F synthase |
3T3-L1 cell |
AKR1B3 mRNA 9 is expressed in preadipocytes, and its level increases about 4fold at day 1 after initiation of adipocyte differentiation, and then quickly decreases the following day to a level lower than that in the preadipocytes |
712494 |
1.11.1.20 | prostamide/prostaglandin F2alpha synthase |
3T3-L1 cell |
- |
740711 |
2.4.1.255 | protein O-GlcNAc transferase |
3T3-L1 cell |
- |
693100 |
3.1.3.48 | protein-tyrosine-phosphatase |
3T3-L1 cell |
- |
729290 |
6.4.1.1 | pyruvate carboxylase |
3T3-L1 cell |
- |
690828, 705517 |
2.7.10.1 | receptor protein-tyrosine kinase |
3T3-L1 cell |
- |
721490 |
2.7.10.1 | receptor protein-tyrosine kinase |
3T3-L1 cell |
HER2 is absent in undifferentiated cultures of adipocytes, not subjected to hormonal treatment but processed at the same time as the differentiating cultures from day 0 to day 14. Expression of HER2 significantly augments during the differentiation of preadipocytes. HER2 acculmulation reaches a maximum at day 9 |
693316 |
3.6.5.2 | small monomeric GTPase |
3T3-L1 cell |
- |
699992, 701352 |
4.1.2.27 | sphinganine-1-phosphate aldolase |
3T3-L1 cell |
- |
681269 |
4.1.2.27 | sphinganine-1-phosphate aldolase |
3T3-L1 cell |
CL 173 (ATCC) |
5119 |
1.14.19.1 | stearoyl-CoA 9-desaturase |
3T3-L1 cell |
- |
687766, 746212 |
1.14.19.1 | stearoyl-CoA 9-desaturase |
3T3-L1 cell |
conjugated linoleic acid decreases SCD1 gene expression |
675626 |
1.14.19.1 | stearoyl-CoA 9-desaturase |
3T3-L1 cell |
isozyme SCD1 is dramatically induced during 3T3L1 differentiation |
746542 |
4.1.1.29 | sulfinoalanine decarboxylase |
3T3-L1 cell |
- |
693792 |
3.1.1.3 | triacylglycerol lipase |
3T3-L1 cell |
- |
707547, 708289 |
3.1.1.3 | triacylglycerol lipase |
3T3-L1 cell |
adipocytes, enzyme expression during differentiation of adipocytes, maximal at day 8, overview |
664719 |
3.4.11.4 | tripeptide aminopeptidase |
3T3-L1 cell |
- |
679509 |
1.17.1.4 | xanthine dehydrogenase |
3T3-L1 cell |
mRNA expression increases 8-fold during the first 12 hr postinduction and subsequently declines to preinduction levels by day 3. Xanthine dehydrogenase activity is maximally induced at 24 hr postinduction, while xanthine oxidase activity remains relatively unchanged |
672979 |
1.14.11.66 | [histone H3]-trimethyl-L-lysine9 demethylase |
3T3-L1 cell |
- |
746217 |
2.7.11.31 | [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase |
3T3-L1 cell |
- |
665636, 702510, 703277 |