1.1.1.146 11beta-hydroxysteroid dehydrogenase 3T3-L1 cell - 686411, 697625, 700121, 700180, 712594, 740806 1.1.1.146 11beta-hydroxysteroid dehydrogenase 3T3-L1 cell differentiation of cells causes a strong increase in 11beta-hydroxysteroid dehydrogenase protein levels, occuring late in the differentiation protocol. Reduction of 11beta-hydroxysteroid dehydrogenase activity in 3T3-L1 fibroblasts, achieved by pharmacological inhibition or adenovirally mediated delivery of short hairpin RNA constructs, specifically blocks the ability of inactive glucocorticoids to drive 3T3-L1 differentiation. Even modest increases in exogenous 11beta-hydroxysteroid dehydrogenase expression in 3T3-L1 fibroblasts, to levels comparable with endogenous 1 11beta-hydroxysteroid dehydrogenase in differentiated 3T3-L1 adipocytes, are sufficient to block adipogenesis 692987 1.1.1.188 prostaglandin-F synthase 3T3-L1 cell AKR1B3 mRNA 9 is expressed in preadipocytes, and its level increases about 4fold at day 1 after initiation of adipocyte differentiation, and then quickly decreases the following day to a level lower than that in the preadipocytes 712494 1.1.1.205 IMP dehydrogenase 3T3-L1 cell - 702061 1.1.1.40 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) 3T3-L1 cell - 739138 1.1.1.44 phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating) 3T3-L1 cell - 685541 1.1.1.8 glycerol-3-phosphate dehydrogenase (NAD+) 3T3-L1 cell - 699948, 760740 1.11.1.20 prostamide/prostaglandin F2alpha synthase 3T3-L1 cell - 740711 1.14.11.4 procollagen-lysine 5-dioxygenase 3T3-L1 cell - 745769 1.14.11.66 [histone H3]-trimethyl-L-lysine9 demethylase 3T3-L1 cell - 746217 1.14.18.6 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase 3T3-L1 cell - 734433 1.14.19.1 stearoyl-CoA 9-desaturase 3T3-L1 cell - 687766, 746212 1.14.19.1 stearoyl-CoA 9-desaturase 3T3-L1 cell conjugated linoleic acid decreases SCD1 gene expression 675626 1.14.19.1 stearoyl-CoA 9-desaturase 3T3-L1 cell isozyme SCD1 is dramatically induced during 3T3L1 differentiation 746542 1.17.1.4 xanthine dehydrogenase 3T3-L1 cell mRNA expression increases 8-fold during the first 12 hr postinduction and subsequently declines to preinduction levels by day 3. Xanthine dehydrogenase activity is maximally induced at 24 hr postinduction, while xanthine oxidase activity remains relatively unchanged 672979 1.2.3.1 aldehyde oxidase 3T3-L1 cell preadipocytes have a very low expression of AOX1, in 2 days differentiated cells AOX1 is induced and is not further upregulated in 3, 7 and 9 days differentiated cells. AOX1 mRNA is nearly four-fold higher in 2 days differentiated cells when compared to preadipocytes and doubles from days 2 to 6 697933 1.3.1.48 13,14-dehydro-15-oxoprostaglandin 13-reductase 3T3-L1 cell - 687562 1.3.3.6 acyl-CoA oxidase 3T3-L1 cell - 671758 1.3.99.23 all-trans-retinol 13,14-reductase 3T3-L1 cell - -, 700994, 763507 1.6.3.1 NAD(P)H oxidase (H2O2-forming) 3T3-L1 cell - 685345 2.1.1.1 nicotinamide N-methyltransferase 3T3-L1 cell elevated enzyme activity during cell differentiation in adipose tissue 701990 2.1.1.17 phosphatidylethanolamine N-methyltransferase 3T3-L1 cell - 719858 2.1.1.348 mRNA m6A methyltransferase 3T3-L1 cell - 757661 2.3.1.108 alpha-tubulin N-acetyltransferase 3T3-L1 cell - 735631 2.3.1.15 glycerol-3-phosphate 1-O-acyltransferase 3T3-L1 cell - 756106, 757868 2.3.1.15 glycerol-3-phosphate 1-O-acyltransferase 3T3-L1 cell Gpat3 mRNA increases 60fold during differentiation of 3T3-L1 preadipocytes to mature adipocytes, suggesting a critical role in adipocytes 701623 2.3.1.15 glycerol-3-phosphate 1-O-acyltransferase 3T3-L1 cell pre- and post-adipocytes 688948 2.3.1.20 diacylglycerol O-acyltransferase 3T3-L1 cell - 758179 2.3.1.20 diacylglycerol O-acyltransferase 3T3-L1 cell 3T3-L1 fibroblasts differentiated into adipocytes 486513 2.3.1.21 carnitine O-palmitoyltransferase 3T3-L1 cell CPT I and CPT II 486577 2.3.1.48 histone acetyltransferase 3T3-L1 cell - 688978 2.3.1.85 fatty-acid synthase system 3T3-L1 cell - 660895 2.3.3.8 ATP citrate synthase 3T3-L1 cell - 736797 2.4.1.11 glycogen(starch) synthase 3T3-L1 cell synthetic peroxisome proliferator-activated receptor beta/delta, and gamma agonists markedly up-regulate Gys-2 mRNA and protein expression in mouse 3T3-L1 adipocytes 686019 2.4.1.255 protein O-GlcNAc transferase 3T3-L1 cell - 693100 2.4.1.80 ceramide glucosyltransferase 3T3-L1 cell - 679436 2.4.2.12 nicotinamide phosphoribosyltransferase 3T3-L1 cell - 735872, 759503 2.4.3.1 beta-galactoside alpha-(2,6)-sialyltransferase 3T3-L1 cell - 759485 2.7.1.137 phosphatidylinositol 3-kinase 3T3-L1 cell - 709772 2.7.1.150 1-phosphatidylinositol-3-phosphate 5-kinase 3T3-L1 cell - 723139 2.7.1.154 phosphatidylinositol-4-phosphate 3-kinase 3T3-L1 cell adipocyte 640921 2.7.1.67 1-phosphatidylinositol 4-kinase 3T3-L1 cell - 676001 2.7.10.1 receptor protein-tyrosine kinase 3T3-L1 cell - 721490 2.7.10.1 receptor protein-tyrosine kinase 3T3-L1 cell HER2 is absent in undifferentiated cultures of adipocytes, not subjected to hormonal treatment but processed at the same time as the differentiating cultures from day 0 to day 14. Expression of HER2 significantly augments during the differentiation of preadipocytes. HER2 acculmulation reaches a maximum at day 9 693316 2.7.11.1 non-specific serine/threonine protein kinase 3T3-L1 cell - 761503 2.7.11.1 non-specific serine/threonine protein kinase 3T3-L1 cell isozyme PKNalpha 662053 2.7.11.11 cAMP-dependent protein kinase 3T3-L1 cell - 694155 2.7.11.24 mitogen-activated protein kinase 3T3-L1 cell - 740424 2.7.11.31 [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase 3T3-L1 cell - 665636, 702510, 703277 2.7.4.21 inositol-hexakisphosphate 5-kinase 3T3-L1 cell - 738392 2.7.4.3 adenylate kinase 3T3-L1 cell - 722732 2.7.7.41 phosphatidate cytidylyltransferase 3T3-L1 cell - 738826 2.8.2.4 estrone sulfotransferase 3T3-L1 cell - -, 725979, 760988 3.1.1.3 triacylglycerol lipase 3T3-L1 cell - 707547, 708289 3.1.1.3 triacylglycerol lipase 3T3-L1 cell adipocytes, enzyme expression during differentiation of adipocytes, maximal at day 8, overview 664719 3.1.1.34 lipoprotein lipase 3T3-L1 cell preadipocyte cell line -, 709009 3.1.1.34 lipoprotein lipase 3T3-L1 cell scopoletin significantly increases lipoprotein lipase activity in 3T3-L1 adipocytes 680239 3.1.1.5 lysophospholipase 3T3-L1 cell - 693213 3.1.1.79 hormone-sensitive lipase 3T3-L1 cell - 714794 3.1.3.1 alkaline phosphatase 3T3-L1 cell activity increases during adipogenesis 677519 3.1.3.4 phosphatidate phosphatase 3T3-L1 cell - 680814, 708998, 709417, 709945, 714316 3.1.3.4 phosphatidate phosphatase 3T3-L1 cell lipin-1 and lipin-2 707555 3.1.3.46 fructose-2,6-bisphosphate 2-phosphatase 3T3-L1 cell - 751121 3.1.3.46 fructose-2,6-bisphosphate 2-phosphatase 3T3-L1 cell adipocyte cell line 664718 3.1.3.48 protein-tyrosine-phosphatase 3T3-L1 cell - 729290 3.1.3.86 phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 3T3-L1 cell - 750315 3.1.4.11 phosphoinositide phospholipase C 3T3-L1 cell - 716113 3.4.11.3 cystinyl aminopeptidase 3T3-L1 cell - -, 669191, 683133, 709666, 754109, 755404 3.4.11.4 tripeptide aminopeptidase 3T3-L1 cell - 679509 3.4.21.46 complement factor D 3T3-L1 cell - 755129 3.4.21.B26 proprotein convertase 5 3T3-L1 cell - 675967 3.4.22.38 cathepsin K 3T3-L1 cell - 695943 3.4.22.38 cathepsin K 3T3-L1 cell ATCC CL-173 697619 3.4.22.52 calpain-1 3T3-L1 cell - 707364 3.4.24.B3 matrix metalloproteinase-11 3T3-L1 cell - 694496 3.5.4.16 GTP cyclohydrolase I 3T3-L1 cell adipocyte cell line 667769 3.6.5.2 small monomeric GTPase 3T3-L1 cell - 699992, 701352 4.1.1.29 sulfinoalanine decarboxylase 3T3-L1 cell - 693792 4.1.1.65 phosphatidylserine decarboxylase 3T3-L1 cell - 715603 4.1.2.27 sphinganine-1-phosphate aldolase 3T3-L1 cell - 681269 4.1.2.27 sphinganine-1-phosphate aldolase 3T3-L1 cell CL 173 (ATCC) 5119 5.2.1.8 peptidylprolyl isomerase 3T3-L1 cell - 715609 5.6.2.2 DNA topoisomerase (ATP-hydrolysing) 3T3-L1 cell - 747492 6.2.1.1 acetate-CoA ligase 3T3-L1 cell marked induction of AceCS1 mRNA and protein during differentiation of 3T3-L1 cells, neither AceCS2 mRNA nor protein is detected in undifferentiated or differentiated 3T3-L1 cells 652101 6.2.1.16 acetoacetate-CoA ligase 3T3-L1 cell - 691065, 727116 6.2.1.3 long-chain-fatty-acid-CoA ligase 3T3-L1 cell - 662593 6.3.4.2 CTP synthase (glutamine hydrolysing) 3T3-L1 cell - 727427 6.4.1.1 pyruvate carboxylase 3T3-L1 cell - 690828, 705517