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3.2.1.24: alpha-mannosidase

This is an abbreviated version!
For detailed information about alpha-mannosidase, go to the full flat file.

Word Map on EC 3.2.1.24

Reaction

2-O-alpha-D-mannopyranosyl-D-mannopyranose
+
H2O
= 2 alpha-D-mannopyranose

Synonyms

1,2-alpha-D-mannosidase, 1,2-alpha-mannosidase, acid alpha-mannosidase, acidic alpha-mannosidase, alpha 1,2-mannosidase, Alpha mannosidase 6A8B, alpha-1,2-mannosidase, alpha-1,3 mannosidase, alpha-1,4 mannosidase, alpha-D-mannopyranosidase, alpha-D-mannosidase, Alpha-D-mannoside mannohydrolase, alpha-man, alpha-mann, alpha-mannosidase, alpha-mannosidase C, alpha-mannosidase E-II, alpha-mannosidase I, alpha-mannosidase IA, alpha-mannosidase II, alpha-mannosidase III, alpha1,2-mannosidase, AMAN, class II alpha-mannosidase, CpGH125, ER-alpha-mannosidase, ER-mannosidase II, exo-alpha-mannosidase, GH38 alpha-mannosidase, GH38 alpha-mannosidase II, GH38 class II alpha-mannosidase, GH38 enzyme, Golgi alpha-mannosidase II, Golgi mannosidase IA, Golgi mannosidase IB, Laman, Lysosomal acid alpha-mannosidase, lysosomal alpha-D-mannosidase, lysosomal alpha-mannosidase, Man1p, MAN2B1, Man2C1, Man2C1 alpha-mannosidase, ManA, mannanase, MGG_00994.6, More, neutral alpha-mannosidase, neutral/cytosol mannosidase, p-nitrophenyl-alpha-mannosidase, SpGH125, SpGH38, Ssa-man, SSO3006, TM1851, TMM, velmanase alfa

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.24 alpha-mannosidase

Purification

Purification on EC 3.2.1.24 - alpha-mannosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography with baker's yeast mannan gel
-
ammonium sulfate precipitation, anion exchange, galactose Sepharose, phenyl Sepharose, gel permeation and Con A Sepharose chromatography
-
catalytic subunit, to homogeneity
-
DEAE-cellulose column chromatography
FLAG–tagged Man2C1 from cytosolic fraction of HEK-293 cells, analysed by silver staining
from amture red fruit
-
from Erythrina indica seeds by gel filtration and affinity chromatography
-
further purification of TAKA-diastase, to homogeneity
-
glutathione S-transferase/mannosidase III fusion protein
-
near homogeneitiy
-
near homogeneity, chromatography steps
near homogeneity, doesn't bind to ConA-Sepharose
-
near homogeneity, preincubation with Co2+, metal chelating affinity chromatography
-
Ni-NTA column chromatography
-
partial
partial, affinity chromatography
-
purification procedure consists of ammonium sulfate precipitation, anion exchange, ConA lectin affinity and gel filtration chromatography
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant protein, immobilized on IgG Sepharose
-
recombinant protein, to homogeneity
recombinant TM1851 from Escherichia coli by ion exchange and hydroxyapatite chromatography, and gel filtration
-
recombinant yeast alpha-factor signal sequence-fusion enzyme from Pichia pastoris strain GS115 by ion exchange and hydrophobic interaction chromatography, eluent is ammonium sulfate, and gel filtration
-
SDS-PAGE, anion-exchange, size exclusion chromatography
separation of neutral mannosidase from acid mannosidase by 1step Co-chelate affinity chromatography
-
to homogeneity, chromatography steps
to homogeneity, chromatography steps, affinity chromatography
-
to homogeneity, chromatography steps, chromatofocusing
-
to homogeneity, chromatography steps, cobalt-chelating Sepharose
-
to homogeneity, chromatography steps, isoelectric focusing
to homogeneity, chromatography steps, preparative electrophoresis
-
to homogeneity, immunoabsorbent chromatography
-
to homogeneity, one step affinity chromatography
-
to homogeneity, preparative PAGE
-
using Ni-NTA chromatography