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Literature summary for 3.2.1.24 extracted from

  • Paus, E.
    Alpha-mannosidase from Phaseolus vulgaris. Composition and structural properties (1977), Eur. J. Biochem., 73, 155-161.
    View publication on PubMed
Search for more literature for 3.2.1.24

Inhibitors

Inhibitors Comment Organism Structure
D-mannono-1,4-lactone competitive inhibition, Ki: 13 mM Phaseolus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
 p-nitrophenyl-alpha-D-mannopyranoside alpha-mannosidase I Phaseolus vulgaris
2
-
 p-nitrophenyl-alpha-D-mannopyranoside alpha-mannosidase II Phaseolus vulgaris

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains 2 mol Zn/mol enzyme Phaseolus vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
220000
-
 gel electrophoresis, sedimentation equilibrium analysis Phaseolus vulgaris

Organism

Organism UniProt Comment Textmining
Phaseolus vulgaris
-
 alpha-mannosidases I and II
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein alpha-mannosidaseI contains mannose and glucose, alpha-mannosidase II mannose, galactose and glucose Phaseolus vulgaris

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, immunoabsorbent chromatography Phaseolus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Phaseolus vulgaris p-nitrophenol + alpha-D-mannopyranose
-
 ?

Subunits

Subunits Comment Organism
dimer denaturating gel electrophoresis, electron microscopy analysis Phaseolus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3 4.5
-
 Phaseolus vulgaris