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3.2.1.24: alpha-mannosidase

This is an abbreviated version!
For detailed information about alpha-mannosidase, go to the full flat file.

Word Map on EC 3.2.1.24

Reaction

2-O-alpha-D-mannopyranosyl-D-mannopyranose
+
H2O
= 2 alpha-D-mannopyranose

Synonyms

1,2-alpha-D-mannosidase, 1,2-alpha-mannosidase, acid alpha-mannosidase, acidic alpha-mannosidase, alpha 1,2-mannosidase, Alpha mannosidase 6A8B, alpha-1,2-mannosidase, alpha-1,3 mannosidase, alpha-1,4 mannosidase, alpha-D-mannopyranosidase, alpha-D-mannosidase, Alpha-D-mannoside mannohydrolase, alpha-man, alpha-mann, alpha-mannosidase, alpha-mannosidase C, alpha-mannosidase E-II, alpha-mannosidase I, alpha-mannosidase IA, alpha-mannosidase II, alpha-mannosidase III, alpha1,2-mannosidase, AMAN, class II alpha-mannosidase, CpGH125, ER-alpha-mannosidase, ER-mannosidase II, exo-alpha-mannosidase, GH38 alpha-mannosidase, GH38 alpha-mannosidase II, GH38 class II alpha-mannosidase, GH38 enzyme, Golgi alpha-mannosidase II, Golgi mannosidase IA, Golgi mannosidase IB, Laman, Lysosomal acid alpha-mannosidase, lysosomal alpha-D-mannosidase, lysosomal alpha-mannosidase, Man1p, MAN2B1, Man2C1, Man2C1 alpha-mannosidase, ManA, mannanase, MGG_00994.6, More, neutral alpha-mannosidase, neutral/cytosol mannosidase, p-nitrophenyl-alpha-mannosidase, SpGH125, SpGH38, Ssa-man, SSO3006, TM1851, TMM, velmanase alfa

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.24 alpha-mannosidase

Specific Activity

Specific Activity on EC 3.2.1.24 - alpha-mannosidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.01
-
in leaf and root
0.02
-
in embryonic axis
0.07
-
enzyme I with 4-methylumbelliferyl-mannopyranoside as substrate
0.09
-
enzyme II with 4-methylumbelliferyl-mannopyranoside as substrate
0.11
-
with p-nitrophenyl-alpha-D-mannopyranoside as substrate
0.29
-
-
0.45
-
in cotyledon
1.93
-
alpha-mannosidase B
11.9
-
-
110.2
-
-
129.2
-
isoforms III
14.4
substrate: 4-nitrophenyl alpha-D-mannoside, pH 6.5, 65°C
15.94
-
alpha-mannosidase A
153
-
-
16.27
-
-
182
-
isoforms II
199.9
-
isoforms I
2.42
-
for Tris-eluted isoform
2.5
-
alpha-mannosidase A
20.52
-
-
21.1
-
-
2200
-
with 4-methylumbelliferyl-alpha-D-mannopyranoside as substrate
4.2
-
purified recombinant TM1851
4.6
-
alpha-mannosidase B
45
-
in the presence of Zn2+
48.5
-
-
6.6
ManA is equally active with mannobiose substrates, released mannose is quantified, resulting in a specific activity of approximately 6.6 U/mg
additional information