Literature summary for 3.2.1.24 extracted from

Tempel, W.; Karaveg, K.; Liu, Z.J.; Rose, J.; Wang, B.C.; Moremen, K.W.
Structure of mouse Golgi alpha-mannosidase IA reveals the molecular basis for substrate specificity among class 1 (family 47 glycosylhydrolase) alpha1,2-mannosidases (2004), J. Biol. Chem., 279, 29774-29786.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of Golgi alpha-mannosidase IA in fusion with the yeast alpha-factor signal sequence in Pichia pastoris strain GS115	Mus musculus

Crystallization (Commentary)

Crystallization (Comment)	Organism
30 mg/ml purified recombinant Golgi alpha-mannosidase IA in 20 mM MES, pH 6.5, 150 mM NaCl, 5 mM CaCl2, and 0.75 M NDSB-201, crystallization of free enzyme by microbatch method using a precipitation solution containing 15-20% PEG 4000 at pH 4.5-6.5, cocrystallization of 1-deoxymannojirimycin bound to the enzyme by hanging drop vapour diffusion method at 37°C, 0.001 ml of a solution containing 200 mM 1-deoxymannojirimycin is mixed with an equal volume of crystallization solution containing 100 mM MES and 100 mM Tris-HCl, pH 6.0, and 25-35% PEG 4000, 2 days at 18°C, X-ray diffraction structure determination and analysis at 1.5 A resolution, structure modelling	Mus musculus

Crystallization (Comment)

Organism

30 mg/ml purified recombinant Golgi alpha-mannosidase IA in 20 mM MES, pH 6.5, 150 mM NaCl, 5 mM CaCl2, and 0.75 M NDSB-201, crystallization of free enzyme by microbatch method using a precipitation solution containing 15-20% PEG 4000 at pH 4.5-6.5, cocrystallization of 1-deoxymannojirimycin bound to the enzyme by hanging drop vapour diffusion method at 37°C, 0.001 ml of a solution containing 200 mM 1-deoxymannojirimycin is mixed with an equal volume of crystallization solution containing 100 mM MES and 100 mM Tris-HCl, pH 6.0, and 25-35% PEG 4000, 2 days at 18°C, X-ray diffraction structure determination and analysis at 1.5 A resolution, structure modelling

Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
1-deoxymannojirimycin	class 1 alpha1,2-mannosidase inhibitor	Mus musculus
kifunensine	class 1 alpha1,2-mannosidase inhibitor	Mus musculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	ER alpha-mannosidase I	Mus musculus	5783	-
Golgi apparatus	Golgi alpha-mannosidase IA	Mus musculus	5794	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	is critical in coordination of the enzyme's (alphaalpha)7 barrel structure, located at the base of the barrel	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
Man8GlcNAc2 + 3 H2O	Mus musculus	Golgi alpha-mannosidase in vivo	Man5GlcNAc2 + 3 D-mannose	-	?
Man9GlcNAc2 + H2O	Mus musculus	endoplasmic reticulum alpha-mannosidase in vivo	Man8GlcNAc2 + mannose	-	?
additional information	Mus musculus	class 1 alpha1,2-mannosidases of glycosylhydrolase family 47 play critical roles in the maturation of Asn-linked glycoproteins in the endoplasmic reticulum and Golgi complex as well as in the recognition and timing of disposal of terminally unfolded proteins by ER-associated degradation, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant yeast alpha-factor signal sequence-fusion enzyme from Pichia pastoris strain GS115 by ion exchange and hydrophobic interaction chromatography, eluent is ammonium sulfate, and gel filtration	Mus musculus

Reaction

Reaction	Comment	Organism	Reaction ID
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O = 2 alpha-D-mannopyranose	active site structure and catalytic residues, e.g. Asn515 involved in substrate binding, reaction mechanism and processing steps, overview	Mus musculus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
Man6GlcNAc2 + H2O	slow reaction of the Golgi alpha-mannosidase in vitro	Mus musculus	Man5GlcNAc2 + mannose	-	?
Man8GlcNAc2 + 3 H2O	Golgi alpha-mannosidase in vivo	Mus musculus	Man5GlcNAc2 + 3 D-mannose	-	?
Man8GlcNAc2 + 3 H2O	slow reaction of the endoplasmic reticulum alpha-mannosidase in vitro	Mus musculus	Man5GlcNAc2 + 3 D-mannose	-	?
Man9GlcNAc2 + 3 H2O	fast reaction of the Golgi alpha-mannosidase in vitro	Mus musculus	Man6GlcNAc2 + 3 mannose	-	?
Man9GlcNAc2 + H2O	endoplasmic reticulum alpha-mannosidase in vivo	Mus musculus	Man8GlcNAc2 + mannose	-	?
Man9GlcNAc2 + H2O	fast reaction of the endoplasmic reticulum alpha-mannosidase in vitro	Mus musculus	Man8GlcNAc2 + mannose	-	?
additional information	class 1 alpha1,2-mannosidases of glycosylhydrolase family 47 play critical roles in the maturation of Asn-linked glycoproteins in the endoplasmic reticulum and Golgi complex as well as in the recognition and timing of disposal of terminally unfolded proteins by ER-associated degradation, overview	Mus musculus	?	-	?

Subunits

Subunits	Comment	Organism
More	structure analysis, the enzyme has an (alphaalpha)7 barrel structure with a coordinating Ca2+ ion, overview	Mus musculus

Synonyms

Synonyms	Comment	Organism
alpha-mannosidase I	-	Mus musculus
alpha-mannosidase IA	-	Mus musculus
alpha1,2-mannosidase	-	Mus musculus
More	the enzyme belongs to the class 1 alpha1,2-mannosidases of glycosylhydrolase family 47	Mus musculus