Inhibitors | Comment | Organism | Structure |
---|---|---|---|
swainsonine | inhibition of both Tris-eluted and unbound isoforms from cobalt-chelating Sepharose, unbound: Ki: 0.028 mM, Tris-eluted: Ki: 0.004 mM | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
p-nitrophenyl-alpha-D-mannopyranoside | Tris-eluted isoform from cobalt-chelating Sepharose | Rattus sp. | |
0.63 | - |
p-nitrophenyl-alpha-D-mannopyranoside | unbound isoform from cobalt-chelating Sepharose | Rattus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus sp. | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activation for Tris-eluted isoform from cobalt-chelating Sepharose | Rattus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
113000 | - |
Tris-eluted isoform from cobalt-chelating Sepharose, gel filtration | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Man9GlcNAc + 3 H2O | Rattus sp. | - |
Man8GlcNAc + Man7GlcNAc + 3 alpha-D-mannopyranose | products from unbound fraction of cobalt-chelating Sepharose | ? | |
Man9GlcNAc + 4 H2O | Rattus sp. | - |
Man5GlcNAc + 4 alpha-D-mannopyranose | end product for Tris-eluted isoform, representing the Man5-oligosaccharide chain of the dolichol pathway formed in the cytosolic compartment during biosynthesis of N-glycosylprotein glycans | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps, cobalt-chelating Sepharose | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | 2 isoforms, unbound and Tris-eluted fractions from cobalt-chelating Sepharose | Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.42 | - |
for Tris-eluted isoform | Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Man9GlcNAc + 3 H2O | - |
Rattus sp. | Man8GlcNAc + Man7GlcNAc + 3 alpha-D-mannopyranose | products from unbound fraction of cobalt-chelating Sepharose | ? | |
Man9GlcNAc + 4 H2O | - |
Rattus sp. | Man5GlcNAc + 4 alpha-D-mannopyranose | end product for Tris-eluted isoform, representing the Man5-oligosaccharide chain of the dolichol pathway formed in the cytosolic compartment during biosynthesis of N-glycosylprotein glycans | ? | |
p-nitrophenyl-alpha-D-mannopyranoside + H2O | different affinities for two isoforms, unbound fraction and Tris-eluted fraction from cobalt-chelating Sepharose | Rattus sp. | p-nitrophenol + alpha-D-mannopyranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 113000, gel filtration, SDS-PAGE | Rattus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | 6.8 | both isoforms, unbound and Tris-eluted fractions from cobalt-chelating Sepharose | Rattus sp. |