Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-D-mannopyranose | 27% inhibition at 10 mM | Rattus sp. | |
D-mannosylamine | 56% inhibition at 1 mM | Rattus sp. | |
Mannan | 33% inhibition at 50 mM | Rattus sp. | |
methyl-alpha-D-glucoside | 23% inhibition at 10 mM | Rattus sp. | |
methyl-alpha-D-mannopyranoside | 41% inhibition at 10 mM | Rattus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi lumen | luminal fluid | Rattus sp. | 5796 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
4 * 115000, SDS-PAGE | Rattus sp. |
460000 | - |
gel filtration | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GlcNAcMan5GlcNAc + H2O | Rattus sp. | low activity | alpha-D-mannopyranose + ? | - |
? | |
glycoprotein + H2O | Rattus sp. | - |
alpha-D-mannopyranose + ? | - |
? | |
Man-glycopeptide + H2O | Rattus sp. | low activity | alpha-D-mannopyranose + ? | - |
? | |
Man5-9GlcNAc + H2O | Rattus sp. | low activity for Man5GlcNAc | alpha-D-mannopyranose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epididymis | cauda epididymis | Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.52 | - |
- |
Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GlcNAcMan5GlcNAc + H2O | low activity | Rattus sp. | alpha-D-mannopyranose + ? | - |
? | |
glycoprotein + H2O | - |
Rattus sp. | alpha-D-mannopyranose + ? | - |
? | |
Man-glycopeptide + H2O | low activity | Rattus sp. | alpha-D-mannopyranose + ? | - |
? | |
Man5-9GlcNAc + H2O | low activity for Man5GlcNAc | Rattus sp. | alpha-D-mannopyranose + ? | - |
? | |
p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Rattus sp. | p-nitrophenol + alpha-D-mannopyranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 115000, SDS-PAGE | Rattus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
4 | 60-70% loss of activity within 24 h | Rattus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | 6.5 | - |
Rattus sp. |