Activating Compound | Comment | Organism | Structure |
---|---|---|---|
lectin | Erythrina indica seed lectin (EiSL) 35% increase alpha-mannosidase in its activity, abolished in presence of lactose | Erythrina variegata |
Protein Variants | Comment | Organism |
---|---|---|
additional information | N-bromosuccinimide added to determine Trp-residues involved in enzyme activity. N-bromosuccinimide modified inactivation is monitored spectrophotometerically | Erythrina variegata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-bromosuccinimide | N-bromosuccinimide modified inactivation, effect of Trp modification on enzyme activity | Erythrina variegata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | alpha mannosidase is Zn2+ dependent | Erythrina variegata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Erythrina variegata | - |
alpha-mannosidase from Erythrina indica has N-terminal amino acid sequence as DTQEN | - |
Purification (Comment) | Organism |
---|---|
from Erythrina indica seeds by gel filtration and affinity chromatography | Erythrina variegata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Erythrina variegata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-D-mannopyranoside + H2O | able to protect enzyme from quenching of fluorescence | Erythrina variegata | 4-nitrophenol + alpha-D-mannopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-mannosidase | - |
Erythrina variegata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Erythrina variegata |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | To determine the energy of activation of alpha-mannosidase (23 kJ/mol), Vmax is determined at various temperatures | Erythrina variegata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.6 | - |
- |
Erythrina variegata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | fluorescence studies over a pH range show tryptophan in highly hydrophobic environment, pH change does not change the tryptophan environment | Erythrina variegata |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
activity is abolished above pH 6.0 | Erythrina variegata |