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Literature summary for 3.2.1.24 extracted from
- Purification and partial characterization of soluble alpha-mannosidase isoforms from sunflower (Helianthus annuus L.) hypocotyls (1989), Plant Sci., 62, 11-19.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | slight inhibition at 1 mM for isoforms I, II | Helianthus annuus |  |
| Ca2+ | slight inhibition at 1 mM | Helianthus annuus | |
| Cu2+ | complete inhibition at 1 mM | Helianthus annuus | |
| EDTA | complete inhibition at 1 mM, addition of 1 mM Zn2+ fully restores activity for isoforms II, to 69% for isoforms I | Helianthus annuus | |
| Fe2+ | complete inhibition at 1 mM | Helianthus annuus | |
| Hg2+ | slight inhibition at 1 mM for isoforms I,II | Helianthus annuus | |
| Mg2+ | slight inhibition at 1 mM | Helianthus annuus | |
| Mn2+ | complete inhibition at 1 mM | Helianthus annuus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.7 | - |
p-nitrophenyl-alpha-D-mannopyranoside | isoforms II | Helianthus annuus | |
| 3.7 | - |
p-nitrophenyl-alpha-D-mannopyranoside | isoforms I | Helianthus annuus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 260000 | - |
gel filtration, isoforms I and II | Helianthus annuus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helianthus annuus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Helianthus annuus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps, chromatofocusing | Helianthus annuus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hypocotyl | three isoforms I, II and III | Helianthus annuus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 129.2 | - |
isoforms III | Helianthus annuus |
| 182 | - |
isoforms II | Helianthus annuus |
| 199.9 | - |
isoforms I | Helianthus annuus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 25 mM sodium phosphate, pH 7.0, 9 months | Helianthus annuus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Helianthus annuus | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Helianthus annuus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | - |
- |
Helianthus annuus |
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