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Literature summary for 3.2.1.24 extracted from

  • Opheim, D.J.; Touster, O.
    Lysosomal alpha-D-mannosidase of rat liver. Purification and comparison with the golgi and cytosolic alpha-D-mannosidases (1978), J. Biol. Chem., 253, 1017-1023.
    View publication on PubMed
Search for more literature for 3.2.1.24

Inhibitors

Inhibitors Comment Organism Structure
alpha-D-mannopyranose competitive inhibition, Ki: 85 mM Rattus sp.
EDTA addition of Zn2+ restores inhibitory effect Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10
-
 p-nitrophenyl-alpha-D-mannopyranoside
-
 Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Rattus sp. 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
335000
-
 gel filtration Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-glucuronidase peptide + H2O Rattus sp.
-
 alpha-D-mannopyranose + ?
-
 ?
GDP-mannosylpyranose + H2O Rattus sp.
-
 alpha-D-mannopyranose + GDP
-
 ?
glycopeptide + H2O Rattus sp. from ovalbumin alpha-D-mannopyranose + ?
-
 ?
mannan + H2O Rattus sp. from yeast alpha-D-mannose + ?
-
 ?
mannopyranosyl-oligosaccharides + H2O Rattus sp. from yeast mannan, hydrolysis of alpha-1,2- and alpha-1,3-linkages alpha-D-mannopyranose + ?
-
 ?
ribonuclease B + H2O Rattus sp.
-
 oligosaccharide + alpha-D-mannopyranose + ?
-
 ?
thyroglobulin + H2O Rattus sp.
-
 alpha-D-mannopyranose + ?
-
 ?
thyroglobulin peptide A + H2O Rattus sp.
-
 alpha-D-mannopyranose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver lysosomal type Rattus sp.
-

Storage Stability

Storage Stability Organism
4°C, 10 mM sodium acetate, pH 6.0, high protein concentration, 1 month Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-glucuronidase peptide + H2O
-
 Rattus sp. alpha-D-mannopyranose + ?
-
 ?
GDP-mannosylpyranose + H2O
-
 Rattus sp. alpha-D-mannosylpyranose + GDP
-
 ?
GDP-mannosylpyranose + H2O
-
 Rattus sp. alpha-D-mannopyranose + GDP
-
 ?
glycopeptide + H2O from ovalbumin Rattus sp. alpha-D-mannopyranose + ?
-
 ?
mannan + H2O from yeast Rattus sp. alpha-D-mannose + ?
-
 ?
mannopyranosyl-oligosaccharides + H2O from yeast mannan, hydrolysis of alpha-1,2- and alpha-1,3-linkages Rattus sp. alpha-D-mannopyranose + ?
-
 ?
methyl-alpha-D-mannopyranoside + H2O
-
 Rattus sp. alpha-D-mannopyranose + methanol
-
 ?
p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Rattus sp. p-nitrophenol + alpha-D-mannopyranose
-
 ?
ribonuclease B + H2O
-
 Rattus sp. oligosaccharide + alpha-D-mannopyranose + ?
-
 ?
thyroglobulin + H2O
-
 Rattus sp. alpha-D-mannopyranose + ?
-
 ?
thyroglobulin peptide A + H2O
-
 Rattus sp. alpha-D-mannopyranose + ?
-
 ?

Synonyms

Synonyms Comment Organism
lysosomal alpha-D-mannosidase
-
 Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6
-
-
 Rattus sp.