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Literature summary for 3.2.1.24 extracted from

  • Shoup, V.A.; Touster, O.
    Purification and characterization of the alpha-D-mannosidase of rat liver cytosol (1976), J. Biol. Chem., 251, 3845-3852.
    View publication on PubMed
Search for more literature for 3.2.1.24

General Stability

General Stability Organism
stable in the presence of Co2+ at 37°C Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
D-mannosylamine competitive inhibition, Ki = 0.007 mM Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 p-nitrophenyl-alpha-D-mannopyranoside
-
 Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus sp. 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 4 * 110000, SDS-PAGE Rattus sp.
372000 420000 sucrose density gradient centrifugation, gel filtration Rattus sp.

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Oxidation Stability

Oxidation Stability Organism
dithiothreitol stabilizes Rattus sp.

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Rattus sp.

Purification (Commentary)

Purification (Comment) Organism
near homogeneitiy Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
110.2
-
-
 Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Rattus sp. p-nitrophenol + alpha-D-mannopyranose
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 110000, SDS-PAGE Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 5.9
-
 Rattus sp.