Cloned (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | inhibits in presence of Co2+ | Saccharolobus solfataricus | |
Ni2+ | inhibits in presence of Co2+ | Saccharolobus solfataricus | |
swainsonine | the enzyme substituted with Zn2+ is considerably less sensitive to swainsonine compared with the Co2+-, Cd2+-, and Mn2+-substituted enzyme | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
4-nitrophenyl alpha-D-mannoside | pH 5.0, 70°C, enzyme in complex with Co2+ | Saccharolobus solfataricus | |
0.39 | - |
4-nitrophenyl alpha-D-mannoside | pH 5.0, 70°C, enzyme in complex with Mn2+ | Saccharolobus solfataricus | |
0.65 | - |
4-nitrophenyl alpha-D-mannoside | pH 5.0, 70°C, enzyme in complex with Zn2+ | Saccharolobus solfataricus | |
0.85 | - |
alpha-D-Man-(1->2)-D-Man | pH 5.0, 70°C, enzyme in complex with Co2+ | Saccharolobus solfataricus | |
0.99 | - |
4-nitrophenyl alpha-D-mannoside | pH 5.0, 70°C, enzyme in complex with Cd2+ | Saccharolobus solfataricus | |
1 | - |
4-nitrophenyl alpha-D-mannoside | pH 5.0, 70°C, untreated enzyme which with no externally added metal ion in the incubation assay | Saccharolobus solfataricus | |
1.16 | - |
alpha-D-Man-(1->3)-D-Man | pH 5.0, 70°C, enzyme in complex with Co2+ | Saccharolobus solfataricus | |
2 | - |
alpha-D-Man-(1->6)-D-Man | pH 5.0, 70°C, enzyme in complex with Co2+ | Saccharolobus solfataricus | |
2 | - |
alpha-D-Man-(1->4)-D-Man | pH 5.0, 70°C, enzyme in complex with Mn2+ | Saccharolobus solfataricus | |
2.3 | - |
alpha-D-Man-(1->2)-D-Man | pH 5.0, 70°C, enzyme in complex with Mn2+ | Saccharolobus solfataricus | |
2.5 | - |
alpha-D-Man-(1->4)-D-Man | pH 5.0, 70°C, enzyme in complex with Co2+ | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | with alpha-1,2-, alpha-1,3-, alpha-1,4-, or alpha-1,6-mannobiose as a substrate, Co2+ is the only metal ion promoting hydrolysis of all substrates. Mn2+, Cd2+, and Zn2+ can substitute to a varying extent | Saccharolobus solfataricus | |
Co2+ | with alpha-1,2-, alpha-1,3-, alpha-1,4-, or alpha-1,6-mannobiose as a substrate, Co2+ is the only metal ion promoting hydrolysis of all substrates. Mn2+, Cd2+, and Zn2+ can substitute to a varying extent | Saccharolobus solfataricus | |
Mn2+ | with alpha-1,2-, alpha-1,3-, alpha-1,4-, or alpha-1,6-mannobiose as a substrate, Co2+ is the only metal ion promoting hydrolysis of all substrates. Mn2+, Cd2+, and Zn2+ can substitute to a varying extent | Saccharolobus solfataricus | |
Zn2+ | with alpha-1,2-, alpha-1,3-, alpha-1,4-, or alpha-1,6-mannobiose as a substrate, Co2+ is the only metal ion promoting hydrolysis of all substrates. Mn2+, Cd2+, and Zn2+ can substitute to a varying extent | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97UK5 | - |
- |
Saccharolobus solfataricus P2 | Q97UK5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-mannoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + alpha-D-mannose | - |
? | |
4-nitrophenyl alpha-D-mannoside + H2O | - |
Saccharolobus solfataricus P2 | 4-nitrophenol + alpha-D-mannose | - |
? | |
alpha-D-Man-(1->2)-D-Man + H2O | - |
Saccharolobus solfataricus | 2 alpha-D-mannose | - |
? | |
alpha-D-Man-(1->2)-D-Man + H2O | - |
Saccharolobus solfataricus P2 | 2 alpha-D-mannose | - |
? | |
alpha-D-Man-(1->3)-D-Man + H2O | - |
Saccharolobus solfataricus | 2 alpha-D-mannose | - |
? | |
alpha-D-Man-(1->3)-D-Man + H2O | - |
Saccharolobus solfataricus P2 | 2 alpha-D-mannose | - |
? | |
alpha-D-Man-(1->4)-D-Man + H2O | - |
Saccharolobus solfataricus | 2 alpha-D-mannose | - |
? | |
alpha-D-Man-(1->4)-D-Man + H2O | - |
Saccharolobus solfataricus P2 | 2 alpha-D-mannose | - |
? | |
alpha-D-Man-(1->6)-D-Man + H2O | - |
Saccharolobus solfataricus | 2 alpha-D-mannose | - |
? | |
alpha-D-Man-(1->6)-D-Man + H2O | - |
Saccharolobus solfataricus P2 | 2 alpha-D-mannose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GH38 class II alpha-mannosidase | - |
Saccharolobus solfataricus |
ManA | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
alpha-D-Man-(1->4)-D-Man | pH 5.0, 70°C, enzyme in complex with Mn2+ | Saccharolobus solfataricus | |
2 | - |
alpha-D-Man-(1->6)-D-Man | pH 5.0, 70°C, enzyme in complex with Co2+ | Saccharolobus solfataricus | |
3.1 | - |
alpha-D-Man-(1->2)-D-Man | pH 5.0, 70°C, enzyme in complex with Mn2+ | Saccharolobus solfataricus | |
4.8 | - |
4-nitrophenyl alpha-D-mannoside | pH 5.0, 70°C, enzyme in complex with Mn2+ | Saccharolobus solfataricus | |
4.9 | - |
alpha-D-Man-(1->3)-D-Man | pH 5.0, 70°C, enzyme in complex with Co2+ | Saccharolobus solfataricus | |
5.5 | - |
alpha-D-Man-(1->2)-D-Man | pH 5.0, 70°C, enzyme in complex with Co2+ | Saccharolobus solfataricus | |
10.4 | - |
alpha-D-Man-(1->4)-D-Man | pH 5.0, 70°C, enzyme in complex with Co2+ | Saccharolobus solfataricus | |
10.5 | - |
4-nitrophenyl alpha-D-mannoside | pH 5.0, 70°C, untreated enzyme which with no externally added metal ion in the incubation assay | Saccharolobus solfataricus | |
12.5 | - |
4-nitrophenyl alpha-D-mannoside | pH 5.0, 70°C, enzyme in complex with Co2+ | Saccharolobus solfataricus | |
13.6 | - |
4-nitrophenyl alpha-D-mannoside | pH 5.0, 70°C, enzyme in complex with Zn2+ | Saccharolobus solfataricus | |
16.1 | - |
4-nitrophenyl alpha-D-mannoside | pH 5.0, 70°C, enzyme in complex with Cd2+ | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.102 | - |
alpha-D-Man-(1->3)-D-Man | pH 5.0, 70°C, enzyme in complex with Zn2+ | Saccharolobus solfataricus | |
0.106 | - |
alpha-D-Man-(1->4)-D-Man | pH 5.0, 70°C, enzyme in complex with Zn2+ | Saccharolobus solfataricus | |
0.185 | - |
alpha-D-Man-(1->3)-D-Man | pH 5.0, 70°C, enzyme in complex with Mn2+ | Saccharolobus solfataricus | |
0.206 | - |
alpha-D-Man-(1->2)-D-Man | pH 5.0, 70°C, enzyme in complex with Zn2+ | Saccharolobus solfataricus | |
0.277 | - |
alpha-D-Man-(1->2)-D-Man | pH 5.0, 70°C, enzyme in complex with Cd2+ | Saccharolobus solfataricus | |
0.28 | - |
alpha-D-Man-(1->3)-D-Man | pH 5.0, 70°C, enzyme in complex with Cd2+ | Saccharolobus solfataricus | |
0.31 | - |
alpha-D-Man-(1->4)-D-Man | pH 5.0, 70°C, enzyme in complex with Cd2+ | Saccharolobus solfataricus | |
0.95 | - |
alpha-D-Man-(1->4)-D-Man | pH 5.0, 70°C, enzyme in complex with Mn2+ | Saccharolobus solfataricus | |
1 | - |
alpha-D-Man-(1->6)-D-Man | pH 5.0, 70°C, enzyme in complex with Co2+ | Saccharolobus solfataricus | |
1.3 | - |
alpha-D-Man-(1->2)-D-Man | pH 5.0, 70°C, enzyme in complex with Mn2+ | Saccharolobus solfataricus | |
4.2 | - |
alpha-D-Man-(1->4)-D-Man | pH 5.0, 70°C, enzyme in complex with Co2+ | Saccharolobus solfataricus | |
4.2 | - |
alpha-D-Man-(1->3)-D-Man | pH 5.0, 70°C, enzyme in complex with Co2+ | Saccharolobus solfataricus | |
6.4 | - |
alpha-D-Man-(1->2)-D-Man | pH 5.0, 70°C, enzyme in complex with Co2+ | Saccharolobus solfataricus | |
10.5 | - |
4-nitrophenyl alpha-D-mannoside | pH 5.0, 70°C, untreated enzyme which with no externally added metal ion in the incubation assay | Saccharolobus solfataricus | |
12 | - |
4-nitrophenyl alpha-D-mannoside | pH 5.0, 70°C, enzyme in complex with Mn2+ | Saccharolobus solfataricus | |
16 | - |
4-nitrophenyl alpha-D-mannoside | pH 5.0, 70°C, enzyme in complex with Cd2+ | Saccharolobus solfataricus | |
21 | - |
4-nitrophenyl alpha-D-mannoside | pH 5.0, 70°C, enzyme in complex with Zn2+ | Saccharolobus solfataricus | |
120 | - |
4-nitrophenyl alpha-D-mannoside | pH 5.0, 70°C, enzyme in complex with Co2+ | Saccharolobus solfataricus |