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Literature summary for 3.2.1.24 extracted from
- Separation, characterization, and specificity of a-mannosidases from Vigna umbellata (1998), Biosci. Biotechnol. Biochem., 62, 613-621.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-deoxymannojirimycin | - |
Vigna umbellata |  |
| EDTA | complete inactivition, Zn2+ restores, Ca2+, Cd2+, Mg2+, Ni2+, Na+ or Cu2+ restore partly | Vigna umbellata | |
| swainsonine | complete inhibition | Vigna umbellata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 329000 | - |
gel filtration | Vigna umbellata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna umbellata | - |
two isoforms | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Vigna umbellata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | more specific towards Man-alpha-1,2-bonds, but also hydrolysis of alpha-1,3- and of alpha-1,6-bonds | Vigna umbellata | ? | - |
? | |
| p-nitrophenyl-alpha-D-mannoside + H2O | - |
Vigna umbellata | 4-nitrophenol + D-mannose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Vigna umbellata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
- |
Vigna umbellata |
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