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Literature summary for 3.2.1.24 extracted from
- Biosynthesis of glycoproteins in Candida albicans: Biochemical characterization of a soluble alpha-mannosidase (1993), FEMS Microbiol. Lett., 106, 321-326.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-deoxymannojirimicin | 67% inhibition at 1 mM when 4-methylumbelliferyl-alpha-D-mannopyranoside is used, 15% inhibition when p-nitrophenyl-alpha-D-mannopyranoside is used as substrates | Candida albicans |  |
| Ca2+ | 53% inhibition at 0.1 mM | Candida albicans | |
| Cu2+ | complete inhibition at 0.1 mM | Candida albicans | |
| EDTA | 37% inhibition at 5 mM | Candida albicans | |
| EGTA | 32% inhibition at 5 mM | Candida albicans | |
| Mn2+ | 58% inhibition at 0.1 mM | Candida albicans | |
| SDS | 85% inhibition at 0.05%, w/v | Candida albicans | |
| swainsonine | 83% inhibition at 0.3 mM if 4-methylumbelliferyl-alpha-D-mannopyranoside is used, hydrolysis of p-nitrophenyl-alpha-D-mannopyranoside only slightly diminished at 0.3 mM | Candida albicans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
4-methylumbelliferyl-alpha-D-mannopyranoside | - |
Candida albicans | |
| 3.3 | - |
p-nitrophenyl-alpha-D-mannopyranoside | - |
Candida albicans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Candida albicans | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 417000 | - |
gel filtration | Candida albicans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Candida albicans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Candida albicans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.11 | - |
with p-nitrophenyl-alpha-D-mannopyranoside as substrate | Candida albicans |
| 2200 | - |
with 4-methylumbelliferyl-alpha-D-mannopyranoside as substrate | Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-D-mannopyranoside + H2O | - |
Candida albicans | 4-methylumbelliferone + alpha-D-mannopyranose | - |
? | |
| p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Candida albicans | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
- |
Candida albicans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
10% loss of activity after 2.5 h | Candida albicans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Candida albicans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
activity lowered by 22% | Candida albicans |
| 6.8 | - |
activity lowered by 27% | Candida albicans |
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