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Literature summary for 3.2.1.24 extracted from

  • Hossain, M.A.; Nakamura, K.; Kimura, Y.
    alpha-mannosidase involved in turnover of plant complex type N-glycans in tomato (Lycopersicum esculentum) fruits (2009), Biosci. Biotechnol. Biochem., 73, 140-146.
    View publication on PubMed
Search for more literature for 3.2.1.24

Inhibitors

Inhibitors Comment Organism Structure
1-deoxy-mannojirimycin complete inhibition Solanum lycopersicum
Co2+
-
 Solanum lycopersicum
Cu2+
-
 Solanum lycopersicum
swainsonine complete inhibition Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
 4-nitrophenyl-alpha-D-mannopyranoside pH 5.0 Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 2 * 70000, L-subunit, + 2 * 47000, S-subunit, SDS-PAGE Solanum lycopersicum
70000
-
 2 * 70000, L-subunit, + 2 * 47000, S-subunit, SDS-PAGE Solanum lycopersicum
230000
-
 gel filtration Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from amture red fruit Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Solanum lycopersicum 4-nitrophenol + alpha-D-mannopyranose
-
 ?
additional information enzyme hydrolyzes alpha-mannosidase linkages from both high-mannose type and plant complex type N-glycan, but prefers a truncated plant complex type structure to high-mannose type N-glycans bearing alpha-(1,2)-mannosyl residues Solanum lycopersicum ?
-
 ?
additional information enzyme is active toward both high-mannose type and truncated plant complex type N-glycans. It prefers truncated plant complex type N-glycans Solanum lycopersicum ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 2 * 70000, L-subunit, + 2 * 47000, S-subunit, SDS-PAGE Solanum lycopersicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 substrate 4-nitrophenyl-alpha-D-mannopyranoside Solanum lycopersicum
6
-
 substrate Man8GlcNAc2-pyridylaminoside Solanum lycopersicum

pH Range

pH Minimum pH Maximum Comment Organism
8
-
 23% of maximum activity Solanum lycopersicum