Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
swainsonine | IC50: 0.00015 mM | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.6 | - |
p-nitrophenyl-alpha-D-mannopyranoside | - |
Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
670000 | - |
gel filtration | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-mannosidosis fibroblasts + H2O | Mus musculus | - |
Manbeta(1-4)GlcNAc + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | O09159 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein, to homogeneity | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | cDNA library | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.86 | - |
- |
Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6 pyridylamino-Man9GlcNAc2 + H2O | stepwise cleavage to Man2GlcNAc structures | Mus musculus | pyridylamino-Man8GlcNAc2 + pyridylamino-Man7GlcNAc2 + pyridylamino-Man6GlcNAc2 + pyridylamino-Man5GlcNAc2 + pyridylamino-Man4GlcNAc2 + pyridylamino-Man3GlcNAc2 + 21 alpha-D-mannopyranose | - |
? | |
alpha-D-mannosidosis fibroblasts + H2O | - |
Mus musculus | Manbeta(1-4)GlcNAc + ? | - |
? | |
p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Mus musculus | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
pyridylamino-Man5GlcNAc + H2O | - |
Mus musculus | pyridylamino-Man4GlcNAc + pyridylamino-Man3-2GlcNAc + alpha-D-mannose | lesser degree of pyridylamino-Man3-2GlcNAc, stepwise cleavage to Man2GlcNAc structures | ? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.3 | 6 | - |
Mus musculus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00015 | - |
IC50: 0.00015 mM | Mus musculus | swainsonine |