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7.2.2.10: P-type Ca2+ transporter

This is an abbreviated version!
For detailed information about P-type Ca2+ transporter, go to the full flat file.

Word Map on EC 7.2.2.10

Reaction

ATP
+
H2O
 +
Ca2+[side 1]
=
ADP
 +
phosphate
 +
Ca2+[side 2]

Synonyms

ACA, ACA2, ACA8, adenosinetriphosphatase, AfPMR1, AtECA1, ATP-consuming plasma membrane calcium pump, ATP-dependent Ca(2+) pump PMR1, ATP-dependent Ca2+ pump PMR1, ATP-dependent calcium ATPase, Atp2b, ATP6, ATPase 2C1, auto-inhibited Ca2+-ATPase, autoinhibited Ca2+-ATPase, Ca(2+)-transporting ATPase, Ca-ATPase, Ca2+ -ATPase, Ca2+ ATPase, Ca2+ ATPase of sarcoplasmic reticulum, Ca2+ pump, Ca2+ pumping ATPase, Ca2+ transport ATPase, Ca2+ transport ATPase of sarco-endoplasmic reticulum, Ca2+,Mn2+-ATPase, Ca2+-ATPase, Ca2+-ATPase 6, Ca2+-ATPase 8, Ca2+-ATPase pump, Ca2+-ATPase, isoform 10, Ca2+-ATPase, isoform 11, Ca2+-ATPase, isoform 12, Ca2+-ATPase, isoform 13, Ca2+-dependent ATPase, Ca2+-Mg2+-ATPase, Ca2+-pumping ATPase, Ca2+-transporting ATPase, Ca2+/Mn2+ ATPase pump, Ca2+/Mn2+ P-type ATPase, Ca2+/Mn2+-ATPase 1, Ca2+/Mn2+-ATPase 2, calcium and manganese transporting ATPase, calcium pump, calcium-dependent ATPase, Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, calcium-transporting ATPase type 2C member 1, CePMR-1, ChkSERCA3, Cod1 protein, Cod1p, EC 3.6.1.3, EC 3.6.1.38, EC 3.6.3.8, eCA, ECA1, ECA2, ECA3, ECA4, endomembrane Ca2+-ATPase, endoplasmic reticulum class 1/2 Ca(2+) ATPase, endosomal Ca2+/Mn2+ pump, ER-type Ca2+-ATPase, Golgi Ca2+-ATPase, HUSSY-28, LCA, LCA1, Mn2+-transporting P-type ATPase, More, P-type ATPase, P-type Ca2+-ATPase, P-type Ca2+/Mn2+ ATPase, P-type Ca2+/Mn2+-ATPase, P-type calcium ATPase, P2A Ca2+ pump, P2A Ca2+-ATPase, P2B Ca2+ pump, P2B Ca2+-ATPase, PCA1, PCMA4b, PfATP6, phosphatase, adenosine tri, plasma membrane ATPase related 1, plasma membrane Ca-ATPase, plasma membrane Ca2+, plasma membrane Ca2+ ATPase, plasma membrane Ca2+ pump 4xb, plasma membrane Ca2+-ATPase, plasma membrane Ca2+-ATPase 1, plasma membrane Ca2+-ATPase 2, plasma membrane Ca2+-ATPase 4, plasma membrane Ca2+-ATPase 4xb, plasma membrane Ca2+-ATPase isoform 4, plasma membrane Ca2+-ATPase isoform ACA8, plasma membrane Ca2+-ATPase-4, plasma membrane Ca2+-pump, plasma membrane calcium ATPase, plasma membrane calcium ATPase 2 calcium pump, plasma membrane calcium pump, plasma-membrane Ca2+-ATPase, plasmalemmal Ca2+ pump isoform 2, plasmalemmal Ca2+-ATPase, PM Ca2+-ATPase, PMCA, PMCA pump, PMCA1, PMCA2, PMCA3, PMCA4, PMCA4a, PMCA4b, PMR1, PMR1-like calcium ATPase, Pmr1p, sarco(endo)plasmic reticulum ATPase, sarco(endo)plasmic reticulum Ca2+ ATPase, sarco(endo)plasmic reticulum Ca2+-ATPase, sarco(endo)plasmic reticulum Ca2+-ATPase 2b, sarco(endo)plasmic reticulum calcium ATPase, sarco-endoplasmic reticulum Ca2+ ATPase, sarco-endoplasmic reticulum Ca2+-ATPase, sarco/endoplasmic reticulum Ca2+ ATPase, sarco/endoplasmic reticulum Ca2+ ATPase 3, sarco/endoplasmic reticulum Ca2+-ATPase, sarco/endoplasmic reticulum calcium ATPase, sarcoendoplasmic reticulum Ca2+ ATPase6, sarcoendoplasmic reticulum Ca2+-ATPase, sarcoendoplasmic reticulum Ca2+-ATPase isoform 1a, sarcoendoplasmic reticulum calcium ATPase, sarcolemmal Ca2+-ATPase, sarcoplasmic reticulum (Ca2+)-ATPase, sarcoplasmic reticulum ATPase, sarcoplasmic reticulum Ca(2+)-ATPase, sarcoplasmic reticulum Ca-ATPase, sarcoplasmic reticulum Ca2+ ATPase, sarcoplasmic reticulum Ca2+ ATPase 1, sarcoplasmic reticulum Ca2+ ATPase 2, sarcoplasmic reticulum Ca2+ ATPase pump, sarcoplasmic reticulum Ca2+-ATPase, sarcoplasmic reticulum Ca2+-ATPase 1, sarcoplasmic reticulum Ca2+-pump ATPase, sarcoplasmic reticulum calcium ATPase, sarcoplasmic reticulum calcium pump, sarcoplasmic reticulum calcium-dependent adenosine triphosphatase, sarcoplasmic-endoplasmic reticulum Ca2+-ATPase, sarcoplasmic/endoplasmic reticulum Ca2+ ATPase, sarcoplasmic/endoplasmic reticulum Ca2+-ATPase, sarcoplasmic/endoplasmic reticulum calcium ATPase, secretory pathway Ca-ATPase, Secretory pathway Ca2+ transporting ATPase, secretory pathway Ca2+-ATPase, secretory pathway Ca2+-ATPase 1, secretory pathway Ca2+/Mn2+-ATPase, SERCA, SERCA 1, SERCA 2, SERCA 3, SERCA pump, SERCA-1, SERCA-like calcium ATPase, SERCA1, SERCA1a, SERCA1b, SERCA2, SERCA2a, SERCA2b, SERCA3, SERCA3a, SERCA3b, SERCA3c, SERCA3d, SERCA3f, SMA1, SPCA, SPCA1, SPCA2, Spf1, SR Ca-ATPase, SR Ca2+-ATPase, Vacuolar Ca2+-ATPase

ECTree

     7 Translocases
         7.2 Catalysing the translocation of inorganic cations
             7.2.2 Linked to the hydrolysis of a nucleoside triphosphate
                7.2.2.10 P-type Ca2+ transporter

Cloned

Cloned on EC 7.2.2.10 - P-type Ca2+ transporter

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of PMR1, string and inducible heat shock promoter to direct high level expression of PMR1 from a multicopy plasmid
-
ECA3 mutant defective in its endogenous Ca21 pumps is expressed in Saccharomyces cerevisiae strain K616
expressed in a Ca2+ transport-deficient yeast mutant
-
expressed in a Saccharomyces cerevisiae triple mutant defective in both a Golgi and vacuolar Ca2+ pump, strain K616 and strain HI227
expressed in COS-1 cells
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-DE3 pLysS cells
expressed in Escherichia coli XL1-Blue cells
-
expressed in HEK cells
-
expressed in HEK-293 cells
expressed in PC-12 cells
-
expressed in Saccharomyces cerevisiae
-
expressed in Saccharomyces cerevisiae mutant strain K616 devoid of endogenous Ca2+-ATPases
expressed in Saccharomyces cerevisiae strain DBY 2062
-
expressed in Saccharomyces cerevisiae strain K616
expressed in Saccharomyces cerevisiae strain YPH 500
-
expressed in Saccharomyces cerevisiae yeast strain W303.1b/Gal4
-
expressed in Sf9 cells
-
expressed in Sf9 insect cells
-
expression of ACA8 residues 40-95, comprising the calmodulin binding site of the enzyme, in Escherichia coli as protein with an N-terminal fusion consisting of a His6 tag, a lipoyl domain and a TEV protease cleavage site
-
expression of C-terminally biotinylated SERCA1a in Saccharomyces cerevisiae strain W3031.b/Gal4, a thrombin cleavage site is inserted
-
expression of wild type and mutant D813A/D818A of L6-7 loop of enzyme in yeast
-
expression of wild-type SERCA2a in insect cell membranes using the baculovirus transfection system, process optimization, co-expression of phospholamban, overview
-
gene Afpmr1, DNA and amino acid sequence determination and analysis, functional expression in Saccharomyces cerevisiae strain K616, functional complementation of deficient growth in EGTA- and MnCl2-containing medium
-
gene ATP2C2, expression pattern, chromosomal localization 16q24.1, the genes ATP2C1 and ATP2C2 present an identical exon/intron layout, overview, functional expression of SPCA2 in COS-1 cell microsomes
gene ATP2C2, isozyme SPCA2, DNA and amino acid sequence determination and analysis, expression in Mn2+-sensitive pmr1 null mutant strain K616 of Saccharomyces cerevisiae, isozyme SPCA2 complements better than isozyme SPCA1
-
gene PM1 homologue, DNA and amino acid sequence determination and analysis
gene PMR1, DNA and amino acid sequence determination and analysis
-
gene pmr1, expression pattern, overexpression of truncated pmr1 in transgenic worms
-
genes ATP2C genes encoding isozymes 1a-d, expression of isozymes 1a-1d using an SV40 vector, in HEK-293 and COS-1 cells
genes ATP2C1 and ATP2C2, transient overexpression of isozymes SPCA1 and SPCA2 in HEK293 cell microsomal membranes
-
isoforms 3a, b and c, transfection in HEK-293 or COS-1 cells using the calcium phosphate precipitation method
-
isolation of gene from the cDNA library
-
mutant enzymes are expressed in Saccharomyces cerevisiae strain K616 (devoid of endogenous Ca2+-ATPases) and His-tagged N-termini of wild type and mutant proteins are expressed in Escherichia coli strain DH5alpha
mutants are expressed in COS-1 cells
-
overexpression of C-terminally biotinylated SERCA1a, with introduced thrombin cleavage site, in Saccharomyces cerevisiae
overexpression of wild-type and mutant SERCA-1s in COS-1 cells membranes using an SV40 vector for transfection
-
PMCA3 DNA and amino acid sequence determination and analysis, phylogenetic tree, expression analysis during molting cycle
SERCA1 expression in COS-1 cells targeted extensively to intracellular membranes using an adenoviral vector. Higher transcription and expression are obtained in the presence of a His6 tag at the amino terminus, as compared with a His6 tag at the carboxyl SERCA terminus, or no tag. Optimization of expression method, overview
-
the enzyme is a product of the yloB gene
-
the pmr1+ gene has strong genetic interactions with pdt1+ gene that encodes the Nramp-related divalent metal transporter
transfection in HEK-293 cells
-
wild-type and mutant enzymes are transfected in COS-1 cells
-