Literature summary for 7.2.2.10 extracted from

Giacometti, S.; Marrano, C.A.; Bonza, M.C.; Luoni, L.; Limonta, M.; De Michelis, M.I.
Phosphorylation of serine residues in the N-terminus modulates the activity of ACA8, a plasma membrane Ca2+-ATPase of Arabidopsis thaliana (2012), J. Exp. Bot., 63, 1215-1224.

Search for more literature for 7.2.2.10

Activating Compound

Activating Compound	Comment	Organism	Structure
Calmodulin	0.001 mM calmodulin stimulates the activity of wild type enzyme by about 300%	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
mutant enzymes are expressed in Saccharomyces cerevisiae strain K616 (devoid of endogenous Ca2+-ATPases) and His-tagged N-termini of wild type and mutant proteins are expressed in Escherichia coli strain DH5alpha	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
S19A	the mutant shows 95% of wild type activity	Arabidopsis thaliana
S19D	the mutant with 163% of wild type activity is deregulated by showing low activation by calmodulin and tryptic cleavage of the N-terminus	Arabidopsis thaliana
S22A	the mutant shows 70% of wild type activity	Arabidopsis thaliana
S22D	the mutant with wild type activity is deregulated by showing low activation by calmodulin and tryptic cleavage of the N-terminus	Arabidopsis thaliana
S27A	the mutant shows 127% of wild type activity	Arabidopsis thaliana
S27D	the mutant with 89% of wild type activity is deregulated by showing low activation by calmodulin and tryptic cleavage of the N-terminus	Arabidopsis thaliana
S29A	the mutant shows 60% of wild type activity	Arabidopsis thaliana
S29D	the mutant shows 64% of wild type activity	Arabidopsis thaliana
S57A	the mutant shows 50% of wild type activity	Arabidopsis thaliana
S57D	the mutant with 120% of wild type activity is deregulated by showing low activation by calmodulin and tryptic cleavage of the N-terminus. The mutant shows 10fold higher affinity towards calmodulin compared to the wild type enzyme	Arabidopsis thaliana
S99A	the mutant shows 78% of wild type activity	Arabidopsis thaliana
S99D	the mutant with 77% of wild type activity shows half of the wild type affinity towards calmodulin	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plasma membrane	-	Arabidopsis thaliana	5886	-

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9LF79	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	-	Arabidopsis thaliana

Purification (Commentary)

Purification (Comment)	Organism
nickel-NTA-agarose column chromatography and glutathione-Sepharose column chromatography	Arabidopsis thaliana

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + Ca2+/out	-	Arabidopsis thaliana	ADP + phosphate + Ca2+/in	-	?

Synonyms

Synonyms	Comment	Organism
ACA8	-	Arabidopsis thaliana
plasma membrane Ca2+-ATPase	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)