Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.2.2.10 extracted from

  • Berrocal, M.; Marcos, D.; Sepulveda, M.R.; Perez, M.; Avila, J.; Mata, A.M.
    Altered Ca2+ dependence of synaptosomal plasma membrane Ca2+-ATPase in human brain affected by Alzheimers disease (2009), FASEB J., 23, 1826-1834.
    View publication on PubMed
Search for more literature for 7.2.2.10

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin
-
 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293 cells Homo sapiens
expressed in HEK-293 cells Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
amyloid beta-peptide the addition of amyloid beta-peptide to normal brain decreases the PMCA activity resulting in the same Ca2+ dependency as that seen in Alzheimer's disease brain, whereas the addition of amyloid beta-peptide to Alzheimer's disease brain has no effect on PMCA activity, in the absence of cholesterol, the level of inhibition of cerebrum PMCA is 60%, but levels of inhibition decreases with increasing concentrations of cholesterol Homo sapiens
amyloid beta-peptide 1-40
-
 Sus scrofa
amyloid beta-peptide 25-35 a maximum inhibition of 75% is observed with 0.0025 mM amyloid beta-peptide 25-35 for PMCA reconstituted in phosphatidylcholine. Homo sapiens
amyloid beta-peptide 25-35 the activity of overexpressed PMCA4 in a microsomal fraction of the HEK-293 cells is inhibited by amyloid beta-peptide 25-35, a slight inhibition of the activity of PMCA3 is observed Sus scrofa
additional information no inhibition of isoforms PMCA4 and PMCA2 is observed with the amyloid beta-peptide 25-35. The addition of 0.0025 mM amyloid beta-peptide 25-35 to Alzheimer's disease brain has no effect on PMCA activity. No effect of amyloid beta-peptides is seen on SERCA or SPCA activity in either control or Alzheimer's disease brains Homo sapiens
additional information no inhibition of isoforms PMCA4 and PMCA2 is observed with the amyloid beta-peptide 25-35, 0.0025 mM amyloid beta-peptide 25-35 has no effect on the activity of PMCA purified from cerebellum Sus scrofa
thapsigargin inhibits SERCA activity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Homo sapiens 5886
-
plasma membrane
-
 Sus scrofa 5886
-
synaptosome
-
 Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on Homo sapiens
Ca2+ dependent on Sus scrofa

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Sus scrofa P23220
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
cerebellum isoform PMCA 2 is more abundant in the cerebellum than in the cerebrum Sus scrofa
-
telencephalon isoform PMCA 4 is more abundant in the cerebrum than in the cerebellum Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Ca2+/cis
-
 Homo sapiens ADP + phosphate + Ca2+/trans
-
 ?
ATP + H2O + Ca2+/cis
-
 Sus scrofa ADP + phosphate + Ca2+/trans
-
 ?

Synonyms

Synonyms Comment Organism
plasma membrane Ca2+-ATPase
-
 Homo sapiens
plasma membrane Ca2+-ATPase
-
 Sus scrofa
PMCA
-
 Homo sapiens
PMCA
-
 Sus scrofa
SERCA
-
 Homo sapiens
SPCA
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0025
-
-
 Sus scrofa amyloid beta-peptide 25-35