Literature summary for 7.2.2.10 extracted from

Jidenko, M.; Lenoir, G.; Fuentes, J.M.; le Maire, M.; Jaxel, C.
Expression in yeast and purification of a membrane protein, SERCA1a, using a biotinylated acceptor domain (2006), Protein Expr. Purif., 48, 32-42.

Search for more literature for 7.2.2.10

Cloned(Commentary)

Cloned (Comment)	Organism
expression of C-terminally biotinylated SERCA1a in Saccharomyces cerevisiae strain W3031.b/Gal4, a thrombin cleavage site is inserted	Oryctolagus cuniculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Oryctolagus cuniculus	16020	-
sarcoplasmic reticulum	-	Oryctolagus cuniculus	16529	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	required	Oryctolagus cuniculus
Mg2+	required	Oryctolagus cuniculus

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant biotinylated SERCA1a 92fold from Saccharomyces cerevisiae light membrane fraction by avidin affinity chromatography, cleavage of the biotin tag by thrombin, and gel filtration	Oryctolagus cuniculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
muscle	-	Oryctolagus cuniculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
9	-	avidin affinity chromatography purified enzyme	Oryctolagus cuniculus

Synonyms

Synonyms	Comment	Organism
SERCA1a	-	Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Oryctolagus cuniculus

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Release 2023.1 (January 2023)