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Literature summary for 7.2.2.10 extracted from

  • Clausen, J.D.; McIntosh, D.B.; Woolley, D.G.; Andersen, J.P.
    Critical interaction of actuator domain residues arginine 174, isoleucine 188, and lysine 205 with modulatory nucleotide in sarcoplasmic reticulum Ca2+-ATPase (2008), J. Biol. Chem., 283, 35703-35714.
    View publication on PubMed
Search for more literature for 7.2.2.10

Cloned(Commentary)

Cloned (Comment) Organism
mutants are expressed in COS-1 cells Oryctolagus cuniculus

Protein Variants

Protein Variants Comment Organism
I188A displays about 30% reduced ATP turnover rate relative to wild type, whereas the ATP turnover rate is reduced by about 80% Oryctolagus cuniculus
I188F the molecular rate of Ca2+-activated ATP hydrolysis at 37°C with 5 mM MgATP is slightly lower (by less than 15%) than that of wild type enzyme, the mutant displays reduced MgATP affinity Oryctolagus cuniculus
K204A the mutant displays around 40% Ca2+ transport, compared with its about 70% rate of ATP turnover relative to the wild type enzyme Oryctolagus cuniculus
K205A the molecular rate of Ca2+-activated ATP hydrolysis at 37°C with 5 mM MgATP slightly lower than that of wild type enzyme Oryctolagus cuniculus
K205E the molecular rate of Ca2+-activated ATP hydrolysis at 37°C with 5 mM MgATP is slightly lower (by less than 15%) than that of wild type enzyme, the mutant displays reduced MgATP affinity Oryctolagus cuniculus
R174A the molecular rate of Ca2+-activated ATP hydrolysis at 37°C with 5 mM MgATP is similar to, or slightly lower than (by less than 15%) that of wild type enzyme, the mutant displays wild type-like MgATP affinity Oryctolagus cuniculus
R174E the mutant displays wild type-like MgATP affinity Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
sarcoplasmic reticulum
-
 Oryctolagus cuniculus 16529
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Ca2+/cis
-
 Oryctolagus cuniculus ADP + phosphate + Ca2+/trans
-
 ?

Synonyms

Synonyms Comment Organism
sarcoplasmic reticulum Ca2+-ATPase
-
 Oryctolagus cuniculus
SERCA1a isoform Oryctolagus cuniculus