Literature summary for 7.2.2.10 extracted from

Maeda, T.; Sugiura, R.; Kita, A.; Saito, M.; Deng, L.; He, Y.; Yabin, L.; Fujita, Y.; Takegawa, K.; Shuntoh, H.; Kuno, T.
Pmr1, a P-type ATPase, and Pdt1, an Nramp homologue, cooperatively regulate cell morphogenesis in fission yeast the importance of Mn2+ homeostasis (2004), Genes Cells, 9, 71-82 .

Search for more literature for 7.2.2.10

Cloned(Commentary)

Cloned (Comment)	Organism
the pmr1+ gene has strong genetic interactions with pdt1+ gene that encodes the Nramp-related divalent metal transporter	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a pmr1 knockout (DELTApmr1) cells exhibiting hypersensitivity to EGTA. Overexpression of pdt1+ gene suppresses the EGTA-sensitive growth defects of DELTApmr1 cells. Although DELTApdt1 cells appear normal in the regular medium, they show round cell morphology similar to that of the DELTApmr1 cells when Mn2+ is removed from the medium. The removal of Mn2+ also exacerbates the round morphology of the DELTApmr1 cells. The DELTApmr1/DELTApdt1 double mutants grow very slowly and shows extremely aberrant cell morphology with round, enlarged and depolarized shape. The addition of Mn2+, but not Ca2+, to the medium completely suppresses the morphological defects, while both Mn2+ and Ca2+ markedly improve the slow growth of the double mutants. Growth of the DELTApmr1/DELTApdt1 double mutants is affected by Mn2+, Ca2+, FK506, and calcineurin overexpression	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi apparatus	-	Saccharomyces cerevisiae	5794	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Saccharomyces cerevisiae
Mn2+	Mn2+ induces accumulation of Pmr1 and Pmc1 mRNAs in a calcineurin/Prz1-dependent manner	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + Ca2+[side 1]	Saccharomyces cerevisiae	-	ADP + phosphate + Ca2+[side 2]	-	?
ATP + H2O + Ca2+[side 1]	Saccharomyces cerevisiae ATCC 204508	-	ADP + phosphate + Ca2+[side 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P13586	-	-
Saccharomyces cerevisiae ATCC 204508	P13586	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + Ca2+[side 1]	-	Saccharomyces cerevisiae	ADP + phosphate + Ca2+[side 2]	-	?
ATP + H2O + Ca2+[side 1]	-	Saccharomyces cerevisiae ATCC 204508	ADP + phosphate + Ca2+[side 2]	-	?

Synonyms

Synonyms	Comment	Organism
More	see also EC 7.2.2.22	Saccharomyces cerevisiae
P-type ATPase	-	Saccharomyces cerevisiae
P-type Ca2+/Mn2+-ATPase	-	Saccharomyces cerevisiae
PMR1	-	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Saccharomyces cerevisiae

Expression

Organism	Comment	Expression
Saccharomyces cerevisiae	addition of Mn2+, as well as Ca2+, to the medium induces pmr1+ gene expression in a calcineurin/Prz1-dependent manner	up

General Information

General Information	Comment	Organism
malfunction	the pmr1 knockout (DELTApmr1) cells exhibit hypersensitivity to EGTA. The EGTA-hypersensitive phenotype of DELTApmr1 leads to the identification of pdt1+ gene, which encodes an Nramp-related metal transporter. The DELTApmr1 cells show round cell morphology. Although DELTApdt1 cells appear normal in the regular medium, they show round cell morphology similar to that of the DELTApmr1 cells when Mn2+ is removed from the medium. The removal of Mn2+ also exacerbates the round morphology of the DELTApmr1 cells. The DELTApmr1/DELTApdt1 double mutants grow very slowly and shows extremely aberrant cell morphology with round, enlarged and depolarized shape. The addition of Mn2+, but not Ca2+, to the medium completely suppresses the morphological defects, while both Mn2+ and Ca2+ markedly improve the slow growth of the double mutants. Growth of the DELTApmr1/DELTApdt1 double mutants is affected by Mn2+, Ca2+, FK506, and calcineurin overexpression	Saccharomyces cerevisiae
physiological function	enzymes Pmr1 and Pdt1 cooperatively regulate cell morphogenesis through the control of Mn2+ homeostasis, and calcineurin functions as a Mn2+ sensor as well as a Mn2+ homeostasis regulator. The pmr1+ gene has strong genetic interactions with pdt1+ gene that encodes the Nramp-related divalent metal transporte. Pmr1 is regulated by the Ca2+/calcineurin/Prz1 pathway	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)