Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant SERCA-1s in COS-1 cells membranes using an SV40 vector for transfection | Gallus gallus |
Crystallization (Comment) | Organism |
---|---|
purified enzyme in 0.02 mM octaethyleneglycol mono-N-dodecylether and 1 mM Ca2+, mixed with 0.03 mM thapsigargin, 0.12 mM 2,5-di-tert-butyl-1,4-dihydroxybenzene, and 3 mM EGTA, dialysis against a buffer consisting of 2.75 M glycerol, 4% PEG 400, 3 mM MgCl2, 0.04 mM 2,5-di-tert-butyl-1,4-dihydroxybenzene, 2.5 mM NaN3, 0.002 mg/ml butylhydroxytoluene, 0.2 mM DTT, 1 mM EGTA, and 20 mM MES, pH 6.1, 1 month, X-ray diffraction structure determination and analysis at 2.4-2.5 A resolution, molecular replacement and structure modelling | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,5-di-tert-butyl-1,4-dihydroxybenzene | inhibitory effects on wild-type and mutant enzymes, overview | Gallus gallus | |
2,5-di-tert-butyl-1,4-dihydroxybenzene | stabilizes the enzyme structure in absence of Ca2+, binding site structure and binding mode involving Asp59 and Pro308, overview | Oryctolagus cuniculus | |
thapsigargin | - |
Gallus gallus | |
thapsigargin | stabilizes the enzyme structure in absence of Ca2+ | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Gallus gallus | 16020 | - |
membrane | - |
Oryctolagus cuniculus | 16020 | - |
sarcoplasmic reticulum | - |
Gallus gallus | 16529 | - |
sarcoplasmic reticulum | - |
Oryctolagus cuniculus | 16529 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Gallus gallus | |
Ca2+ | binding involves Glu309 which acts as a gating residue for cytoplasmic Ca2+ to reach the binding cavity, Ca2+ binding site structure and mechanism | Oryctolagus cuniculus | |
Mg2+ | - |
Oryctolagus cuniculus | |
Mg2+ | required | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Ca2+/cis | Gallus gallus | - |
ADP + phosphate + Ca2+/trans | - |
? | |
ATP + H2O + Ca2+/cis | Oryctolagus cuniculus | - |
ADP + phosphate + Ca2+/trans | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Oryctolagus cuniculus | P04191 | - |
- |
Purification (Comment) | Organism |
---|---|
partially, recombinant enzyme in microsomes of transfected COS-1 cells | Gallus gallus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + H2O + Ca2+[side 1] = ADP + phosphate + Ca2+[side 2] | Ca2+ binding site structure and mechanism | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Oryctolagus cuniculus | - |
skeletal muscle | fast muscle | Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Ca2+-independent ATPase activity | Gallus gallus | ADP + phosphate | - |
? | |
ATP + H2O + Ca2+/cis | - |
Gallus gallus | ADP + phosphate + Ca2+/trans | - |
? | |
ATP + H2O + Ca2+/cis | - |
Oryctolagus cuniculus | ADP + phosphate + Ca2+/trans | - |
? | |
ATP + H2O + Ca2+/cis | Ca2+-dependent ATPase activity | Gallus gallus | ADP + phosphate + Ca2+/trans | - |
? | |
ATP + H2O + Ca2+/cis | the enzyme performs ATP-dependent Ca2+/H+ antiport and Ca2+-dependent ATPase activity | Oryctolagus cuniculus | ADP + phosphate + Ca2+/trans | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SERCA-1 | - |
Gallus gallus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Gallus gallus |