Literature summary for 7.2.2.10 extracted from

David-Bosne, S.; Florent, I.; Lund-Winther, A.M.; Hansen, J.B.; Buch-Pedersen, M.; Machillot, P.; le Maire, M.; Jaxel, C.
Antimalarial screening via large-scale purification of Plasmodium falciparum Ca2+-ATPase 6 and in vitro studies (2013), FEBS J., 280, 5419-5429.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Saccharomyces cerevisiae yeast strain W303.1b/Gal4	Plasmodium falciparum

Inhibitors

Inhibitors	Comment	Organism	Structure
(2Z)-2-cyano-3-(3,4-dihydroxyphenyl)prop-2-enethioamide	-	Plasmodium falciparum
2,3-bis[(2-hydroxyethyl)sulfanyl]naphthalene-1,4-dione	-	Plasmodium falciparum
2,5-di(tert-butyl)-1,4-benzohydroquinone	-	Plasmodium falciparum
2-phenyl-1,2-benzoselenazol-3(2H)-one	-	Plasmodium falciparum
2-[(cyclopropylcarbonyl)amino]-4,5-dimethylthiophene-3-carboxamide	-	Plasmodium falciparum
4,5-dimethyl-2-([[5-methyl-2-(propan-2-yl)phenoxy]acetyl]amino)thiophene-3-carboxamide	-	Plasmodium falciparum
5-chloro-N-(3,5-dimethoxyphenyl)-4-(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-2-methoxybenzamide	-	Plasmodium falciparum
6-amino-2-(hex-1-yn-1-yl)-9H-purin-9-yl-beta-D-N-ethylribofuranoic amide	-	Plasmodium falciparum
cyclopiazonic acid	very potent inhibitor	Plasmodium falciparum
methyl [(4-tert-butylbenzoyl)amino]acetate	-	Plasmodium falciparum
thapsigargin	-	Plasmodium falciparum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	-	Plasmodium falciparum	5783	-
sarcoplasmic reticulum	-	Plasmodium falciparum	16529	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
139000	-	x * 139000, SDS-PAGE	Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + Ca2+[side 1]	Plasmodium falciparum	-	ADP + phosphate + Ca2+[side 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
streptavidin-Sepharose resin column chromatography	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + Ca2+[side 1]	-	Plasmodium falciparum	ADP + phosphate + Ca2+[side 2]	-	?

Subunits

Subunits	Comment	Organism
?	x * 139000, SDS-PAGE	Plasmodium falciparum

Synonyms

Synonyms	Comment	Organism
ATP6	-	Plasmodium falciparum
Ca2+-ATPase 6	-	Plasmodium falciparum
SERCA	-	Plasmodium falciparum

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0004	-	at pH 7.5 and 25°C	Plasmodium falciparum	cyclopiazonic acid
0.001	-	at pH 7.5 and 25°C	Plasmodium falciparum	2-phenyl-1,2-benzoselenazol-3(2H)-one
0.001	-	at pH 7.5 and 25°C	Plasmodium falciparum	2,3-bis[(2-hydroxyethyl)sulfanyl]naphthalene-1,4-dione
0.0011	-	at pH 7.5 and 25°C	Plasmodium falciparum	4,5-dimethyl-2-([[5-methyl-2-(propan-2-yl)phenoxy]acetyl]amino)thiophene-3-carboxamide
0.0012	-	at pH 7.5 and 25°C	Plasmodium falciparum	methyl [(4-tert-butylbenzoyl)amino]acetate
0.003	-	at pH 7.5 and 25°C	Plasmodium falciparum	5-chloro-N-(3,5-dimethoxyphenyl)-4-(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-2-methoxybenzamide
0.0031	-	at pH 7.5 and 25°C	Plasmodium falciparum	2-[(cyclopropylcarbonyl)amino]-4,5-dimethylthiophene-3-carboxamide
0.007	-	at pH 7.5 and 25°C	Plasmodium falciparum	(2Z)-2-cyano-3-(3,4-dihydroxyphenyl)prop-2-enethioamide
0.0085	-	at pH 7.5 and 25°C	Plasmodium falciparum	6-amino-2-(hex-1-yn-1-yl)-9H-purin-9-yl-beta-D-N-ethylribofuranoic amide
0.065	-	at pH 7.5 and 25°C	Plasmodium falciparum	2,5-di(tert-butyl)-1,4-benzohydroquinone
0.15	-	at pH 7.5 and 25°C	Plasmodium falciparum	thapsigargin

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Release 2023.1 (January 2023)