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7.2.2.10: P-type Ca2+ transporter

This is an abbreviated version!
For detailed information about P-type Ca2+ transporter, go to the full flat file.

Word Map on EC 7.2.2.10

Reaction

ATP
+
H2O
 +
Ca2+[side 1]
=
ADP
 +
phosphate
 +
Ca2+[side 2]

Synonyms

ACA, ACA2, ACA8, adenosinetriphosphatase, AfPMR1, AtECA1, ATP-consuming plasma membrane calcium pump, ATP-dependent Ca(2+) pump PMR1, ATP-dependent Ca2+ pump PMR1, ATP-dependent calcium ATPase, Atp2b, ATP6, ATPase 2C1, auto-inhibited Ca2+-ATPase, autoinhibited Ca2+-ATPase, Ca(2+)-transporting ATPase, Ca-ATPase, Ca2+ -ATPase, Ca2+ ATPase, Ca2+ ATPase of sarcoplasmic reticulum, Ca2+ pump, Ca2+ pumping ATPase, Ca2+ transport ATPase, Ca2+ transport ATPase of sarco-endoplasmic reticulum, Ca2+,Mn2+-ATPase, Ca2+-ATPase, Ca2+-ATPase 6, Ca2+-ATPase 8, Ca2+-ATPase pump, Ca2+-ATPase, isoform 10, Ca2+-ATPase, isoform 11, Ca2+-ATPase, isoform 12, Ca2+-ATPase, isoform 13, Ca2+-dependent ATPase, Ca2+-Mg2+-ATPase, Ca2+-pumping ATPase, Ca2+-transporting ATPase, Ca2+/Mn2+ ATPase pump, Ca2+/Mn2+ P-type ATPase, Ca2+/Mn2+-ATPase 1, Ca2+/Mn2+-ATPase 2, calcium and manganese transporting ATPase, calcium pump, calcium-dependent ATPase, Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, calcium-transporting ATPase type 2C member 1, CePMR-1, ChkSERCA3, Cod1 protein, Cod1p, EC 3.6.1.3, EC 3.6.1.38, EC 3.6.3.8, eCA, ECA1, ECA2, ECA3, ECA4, endomembrane Ca2+-ATPase, endoplasmic reticulum class 1/2 Ca(2+) ATPase, endosomal Ca2+/Mn2+ pump, ER-type Ca2+-ATPase, Golgi Ca2+-ATPase, HUSSY-28, LCA, LCA1, Mn2+-transporting P-type ATPase, More, P-type ATPase, P-type Ca2+-ATPase, P-type Ca2+/Mn2+ ATPase, P-type Ca2+/Mn2+-ATPase, P-type calcium ATPase, P2A Ca2+ pump, P2A Ca2+-ATPase, P2B Ca2+ pump, P2B Ca2+-ATPase, PCA1, PCMA4b, PfATP6, phosphatase, adenosine tri, plasma membrane ATPase related 1, plasma membrane Ca-ATPase, plasma membrane Ca2+, plasma membrane Ca2+ ATPase, plasma membrane Ca2+ pump 4xb, plasma membrane Ca2+-ATPase, plasma membrane Ca2+-ATPase 1, plasma membrane Ca2+-ATPase 2, plasma membrane Ca2+-ATPase 4, plasma membrane Ca2+-ATPase 4xb, plasma membrane Ca2+-ATPase isoform 4, plasma membrane Ca2+-ATPase isoform ACA8, plasma membrane Ca2+-ATPase-4, plasma membrane Ca2+-pump, plasma membrane calcium ATPase, plasma membrane calcium ATPase 2 calcium pump, plasma membrane calcium pump, plasma-membrane Ca2+-ATPase, plasmalemmal Ca2+ pump isoform 2, plasmalemmal Ca2+-ATPase, PM Ca2+-ATPase, PMCA, PMCA pump, PMCA1, PMCA2, PMCA3, PMCA4, PMCA4a, PMCA4b, PMR1, PMR1-like calcium ATPase, Pmr1p, sarco(endo)plasmic reticulum ATPase, sarco(endo)plasmic reticulum Ca2+ ATPase, sarco(endo)plasmic reticulum Ca2+-ATPase, sarco(endo)plasmic reticulum Ca2+-ATPase 2b, sarco(endo)plasmic reticulum calcium ATPase, sarco-endoplasmic reticulum Ca2+ ATPase, sarco-endoplasmic reticulum Ca2+-ATPase, sarco/endoplasmic reticulum Ca2+ ATPase, sarco/endoplasmic reticulum Ca2+ ATPase 3, sarco/endoplasmic reticulum Ca2+-ATPase, sarco/endoplasmic reticulum calcium ATPase, sarcoendoplasmic reticulum Ca2+ ATPase6, sarcoendoplasmic reticulum Ca2+-ATPase, sarcoendoplasmic reticulum Ca2+-ATPase isoform 1a, sarcoendoplasmic reticulum calcium ATPase, sarcolemmal Ca2+-ATPase, sarcoplasmic reticulum (Ca2+)-ATPase, sarcoplasmic reticulum ATPase, sarcoplasmic reticulum Ca(2+)-ATPase, sarcoplasmic reticulum Ca-ATPase, sarcoplasmic reticulum Ca2+ ATPase, sarcoplasmic reticulum Ca2+ ATPase 1, sarcoplasmic reticulum Ca2+ ATPase 2, sarcoplasmic reticulum Ca2+ ATPase pump, sarcoplasmic reticulum Ca2+-ATPase, sarcoplasmic reticulum Ca2+-ATPase 1, sarcoplasmic reticulum Ca2+-pump ATPase, sarcoplasmic reticulum calcium ATPase, sarcoplasmic reticulum calcium pump, sarcoplasmic reticulum calcium-dependent adenosine triphosphatase, sarcoplasmic-endoplasmic reticulum Ca2+-ATPase, sarcoplasmic/endoplasmic reticulum Ca2+ ATPase, sarcoplasmic/endoplasmic reticulum Ca2+-ATPase, sarcoplasmic/endoplasmic reticulum calcium ATPase, secretory pathway Ca-ATPase, Secretory pathway Ca2+ transporting ATPase, secretory pathway Ca2+-ATPase, secretory pathway Ca2+-ATPase 1, secretory pathway Ca2+/Mn2+-ATPase, SERCA, SERCA 1, SERCA 2, SERCA 3, SERCA pump, SERCA-1, SERCA-like calcium ATPase, SERCA1, SERCA1a, SERCA1b, SERCA2, SERCA2a, SERCA2b, SERCA3, SERCA3a, SERCA3b, SERCA3c, SERCA3d, SERCA3f, SMA1, SPCA, SPCA1, SPCA2, Spf1, SR Ca-ATPase, SR Ca2+-ATPase, Vacuolar Ca2+-ATPase

ECTree

     7 Translocases
         7.2 Catalysing the translocation of inorganic cations
             7.2.2 Linked to the hydrolysis of a nucleoside triphosphate
                7.2.2.10 P-type Ca2+ transporter

Crystallization

Crystallization on EC 7.2.2.10 - P-type Ca2+ transporter

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant complex between calmodulin and the regulatory domain of the plasma-membrane Ca2+-ATPase ACA8, mixing of 0.0006 ml of protein solution, containing 16 mg/ml protein in 25 mM Tris, pH 7.0, 50 mM NaCl, 10 mM 2-mercaptoethanol, 5 mM CaCl2, with 0.001 ml reservoir solution, containing 2.0 M ammonium sulfate, 0.1 M CAPS, pH 10.5, 0.2 M lithium sulfate, at final pH 8.2, ar 20°C, X-ray diffraction structure determination and analysis at 3.0 A resolution
-
hanging drop vapor diffusion method, using 220 mM sodium acetate, 4% (v/v) tert-butanol, 5 mM 2-mercaptoethanol, 15% (v/v) glycerol, and 6-7% (w/v) PEG 6000
crystal structure
hanging drop vapor diffusion method, using 220 mM sodium acetate, 4% (v/v) tert-butanol, 5 mM 2-mercaptoethanol, 15% (v/v) glycerol, and 6-7% (w/v) PEG 6000
-
purified enzyme in 0.02 mM octaethyleneglycol mono-N-dodecylether and 1 mM Ca2+, mixed with 0.03 mM thapsigargin, 0.12 mM 2,5-di-tert-butyl-1,4-dihydroxybenzene, and 3 mM EGTA, dialysis against a buffer consisting of 2.75 M glycerol, 4% PEG 400, 3 mM MgCl2, 0.04 mM 2,5-di-tert-butyl-1,4-dihydroxybenzene, 2.5 mM NaN3, 0.002 mg/ml butylhydroxytoluene, 0.2 mM DTT, 1 mM EGTA, and 20 mM MES, pH 6.1, 1 month, X-ray diffraction structure determination and analysis at 2.4-2.5 A resolution, molecular replacement and structure modelling
purified enzyme in complex with purified recombinant phospholamban, from 20 mM imidazole, pH 7.4, 100 mM KCl, 35 mM MgCl2, 0.5 mM EGTA, 0.25 mM Na3VO4, 0.03 mM thapsigargin, three freeze-thaw cycles using liquid nitrogen and thawing in hand, reconstitution at 4°C for several days to 1 week, analysis of interactions between Ca2+-ATPase and the pentameric form of phospholamban in two-dimensional co-crystals, overview
purified enzyme is crystallized preserving natural and essential lipids, high resolution crystal structure determination and analysis, crystallization method optimization
purified recombinant enzyme, hanging drop vapour diffusion method, 12 mg/ml protein in a solution containing 10 mM Ca2+, 1 mm beta,gamma-methyleneadenosine 5'-triphosphate, and 1,2-dioleoyl-sn-glycero-3-phosphocholine, 0.002 ml is mixed with 0.002 ml well solution containing 0.2 M sodium acetate, 10-14% w/v PEG 6000, 10% glycerol, and 4% tert-butanol, crystals grow within 1 to 14 days at 19°C, X-ray diffraction structure determination and analysis at 3.3 A resolution