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ammonium sulfate fractionation, PK agarose bead chromatography, TALON Co2+-affinity resin column chromatography, and TALON Superflow metal affinity resin column chromatography
ammonium sulfate precipitation and Mono-Q column chromatography
ammonium sulfate precipitation, ultrafiltration, and Sepharose Q column chromatography
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based on t-butyloxycarbonyl-L-Leu-bromomethyl ketone affinity chromatography
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extracellular enzyme to homogeneity by ion exchange and hydrophobic interaction chromatography, or by heat treatment at 70°C
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from inclusion bodies, immobilized metal ion affinity chromatography (Ni2+), purity of 95%
heat denaturation, ammonium precipitation and DEAE-cellulose chromatography
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native and recombinant enzymes are purified by Ni-NTA column chromatography, Superdex 200 gel filtration and bestatin-affinity chromatography
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native API and APII to homogeneity by hydrophobic interaction and ion exchange chromatography, and gel filtration
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native enzyme 164fold to homogeneity by ammonium sulfate fractionation, ion exchange and hydroxyapatite chromatography, again ion exchange chromatography, and gel filtration, followed by again ion exchange and hydroxyapatite chromatography
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native enzyme 32fold to homogeneity by anion exchange chromatography and gel filtration
native enzyme from crude cell extract, and recombinant enzyme from overexpressing cells, by anion exchange and hydrophobic interaction chromatography, and gel filtration
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native periplasmic enzyme by ion exchange and hydrophobic interaction chromatography, recombinant MBP-fusion enzyme and recombinant extracellular or mature intracellular wild-type enzyme from Escherichia coli by amylose affinity chromatography and ultrafiltration, and by ammonium sulfate fractionation, dialysis, hydrophobic interaction and anion exchange chromatography, and a second hydrophobic interaction chromatography step
Ni2+-NTA resin chromatography
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Q-Sepharose column chromatography and Superdex 200 gel filtration
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recombinant chimeric enzyme 20.4fold from Escherichia coli strain M15 by an affinity adsorption chromatographic step on a raw starch resin, screening of different elution methods, overview
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recombinant enzyme 5fold from Escherichia coli by ion exchange chromatography
recombinant enzyme from Escherichia coli by cation exchange chromatography and gel filtration
recombinant enzyme from Escherichia coli by ion exchange chromatography
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recombinant enzyme partially from Escherichia coli
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recombinant extracellular enzyme 8.4fold from Pichia pastoris strain GS115 culture supernatant by dialysis, anion exchange chromatography, ultrafiltration, and gel filtration
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain M15 by nickel affinity chromatography
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recombinant His-tagged wild-type and mutant LAPII from Escherichia coli by nickel chelate affinity chromatography
recombinant wild-type and modified Lys-tagged enzymes from Escherichia coli strain M15
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recombinant wild-type and mutant E151H enzymes from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, dialysis, hydrophobic interaction and anion exchange chromatography
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, dialysis, hydrophobic interaction and anion exchange chromatography
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using ion-exchange chromatography, ultrafiltration and gel filtration
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using Ni-NTA chromatography
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using Ni-NTA chromatography
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using Ni-NTA chromatography
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