Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Escherichia coli | |
bestatin | - |
Escherichia coli | |
pepstatin | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
Gly-7-amido-4-methylcoumarin | pH 8.0, 37°C | Escherichia coli | |
0.18 | - |
L-Leu-7-amido-4-methylcoumarin | pH 8.0, 37°C | Escherichia coli | |
0.22 | - |
L-Lys-4-nitroanilide | pH 8.0, 37°C | Escherichia coli | |
0.24 | - |
L-Met-7-amido-4-methylcoumarin | pH 8.0, 37°C | Escherichia coli | |
0.26 | - |
L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin | pH 8.0, 37°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
340000 | - |
SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
heat denaturation, ammonium precipitation and DEAE-cellulose chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.67 | - |
value about, substrate L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin | Escherichia coli |
1.67 | - |
value about, substrate L-Asp-7-amido-4-methylcoumarin | Escherichia coli |
1.67 | - |
value about, substrate L-Gln-7-amido-4-methylcoumarin | Escherichia coli |
1.67 | - |
value about, substrate L-Thr-7-amido-4-methylcoumarin | Escherichia coli |
1.67 | - |
value about, substrate L-Trp-7-amido-4-methylcoumarin | Escherichia coli |
4.17 | - |
value about, substrate L-Pro-7-amido-4-methylcoumarin | Escherichia coli |
8.3 | - |
value about, substrate L-Tyr-7-amido-4-methylcoumarin | Escherichia coli |
25 | - |
value about, substrate Gly-7-amido-4-methylcoumarin | Escherichia coli |
33.3 | - |
value about, substrate L-Leu-7-amido-4-methylcoumarin | Escherichia coli |
33.3 | - |
value about, substrate L-Lys-4-nitroanilide | Escherichia coli |
33.3 | - |
value about, substrate L-Met-7-amido-4-methylcoumarin | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | assay at pH 8.0, 37°C | Escherichia coli | ? | - |
? | |
Gly-7-amido-4-methylcoumarin + H2O | assay at pH 8.0, 37°C | Escherichia coli | 7-amino-4-methylcoumarin + L-Gly | - |
? | |
L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin + H2O | assay at pH 8.0, 37°C | Escherichia coli | 7-amino-4-methylcoumarin + L-Ala-L-Ala-L-Phe | - |
? | |
L-Asp-7-amido-4-methylcoumarin + H2O | assay at pH 8.0, 37°C | Escherichia coli | 7-amino-4-methylcoumarin + L-Asp | - |
? | |
L-Gln-7-amido-4-methylcoumarin + H2O | assay at pH 8.0, 37°C | Escherichia coli | 7-amino-4-methylcoumarin + L-Gln | - |
? | |
L-Leu-7-amido-4-methylcoumarin + H2O | assay at pH 8.0, 37°C | Escherichia coli | 7-amino-4-methylcoumarin + L-Leu | - |
? | |
L-Lys-4-nitroanilide + H2O | assay at pH 8.0, 37°C | Escherichia coli | 4-nitroaniline + L-Lys | - |
? | |
L-Met-7-amido-4-methylcoumarin + H2O | assay at pH 8.0, 37°C | Escherichia coli | 7-amino-4-methylcoumarin + L-Met | - |
? | |
L-Pro-7-amido-4-methylcoumarin + H2O | assay at pH 8.0, 37°C | Escherichia coli | 7-amino-4-methylcoumarin + L-Pro | - |
? | |
L-Thr-7-amido-4-methylcoumarin + H2O | assay at pH 8.0, 37°C | Escherichia coli | 7-amino-4-methylcoumarin + L-Thr | - |
? | |
L-Trp-7-amido-4-methylcoumarin + H2O | assay at pH 8.0, 37°C | Escherichia coli | 7-amino-4-methylcoumarin + L-Trp | - |
? | |
L-Tyr-7-amido-4-methylcoumarin + H2O | assay at pH 8.0, 37°C | Escherichia coli | 7-amino-4-methylcoumarin + L-Tyr | - |
? | |
oxidized insulin B chain + H2O | assay at pH 8.0, 37°C | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
PepA | - |
Escherichia coli |
Peptidase A | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
61 | - |
melting temperature | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin | pH 8.0, 37°C | Escherichia coli | |
11 | - |
Gly-7-amido-4-methylcoumarin | pH 8.0, 37°C | Escherichia coli | |
17 | - |
L-Leu-7-amido-4-methylcoumarin | pH 8.0, 37°C | Escherichia coli | |
17.7 | - |
L-Lys-4-nitroanilide | pH 8.0, 37°C | Escherichia coli | |
20 | - |
L-Met-7-amido-4-methylcoumarin | pH 8.0, 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34 | - |
L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin | pH 8.0, 37°C | Escherichia coli | |
68 | - |
Gly-7-amido-4-methylcoumarin | pH 8.0, 37°C | Escherichia coli | |
81 | - |
L-Met-7-amido-4-methylcoumarin | pH 8.0, 37°C | Escherichia coli | |
82 | - |
L-Lys-4-nitroanilide | pH 8.0, 37°C | Escherichia coli | |
91 | - |
L-Leu-7-amido-4-methylcoumarin | pH 8.0, 37°C | Escherichia coli |