3.4.11.10: bacterial leucyl aminopeptidase
This is an abbreviated version!
For detailed information about bacterial leucyl aminopeptidase, go to the full flat file.
Reaction
release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids
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Synonyms
AAP, Aeromonas proteolytica aminopeptidase, Aminopeptidase, aminopeptidase A, aminopeptidase A (bacteria), aminopeptidase Ap1, aminopeptidase II, AP-II, API, APII, AVP, bacterial leucine aminopeptidase, bacterial M17 aminopeptidase, BSAP, Bsu aminopeptidase, BsuAP, CGase, cysteinylglycinase, double-zinc aminopeptidase, extracellular aminopeptidase, FgLAP, HpM17AP, LAP, LAPII, leucine aminopeptidase, leucine aminopeptidase II, leucine APN, Leucyl aminopeptidase, M17 aminopeptidase, M17 metallo-aminopeptidase, More, MtLAP, PepA, Peptidase A, pepZ, PhpA, ribosomal-bound aminopeptidase, rLAP55, Rv2213, SSAP, TAP, TH-2, thermophilic aminopeptidase, thermostable leucine aminopeptidase, Vibrio aminopeptidase, VpAP, ywaD
ECTree
Temperature Stability
Temperature Stability on EC 3.4.11.10 - bacterial leucyl aminopeptidase
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100
purified enzyme, 10 min, complete inactivation
25 - 50
purified enzyme, completely stable for 240 min
30 - 60
-
recombinant protein
50 - 60
-
preheating of recombinant LAP at 50°C produces the maximum specific activity while at higher temperatures (above 60°C) the enzyme results inactivated
30 - 70
purified recombinant enzyme, stable at 30-60°C. The enzyme retains 63.5% activity after incubation at 70°C for 1 h, and 75% activity after 3 h at 30-60°C
30 - 70
-
stability range, profiles of free and immobilized recombinant wild-type and mutant enzymes, overview
60
purified enzyme, no loss activity within 10 min, 240 min: loss of 12.5% activity
60
-
32 min, 50% remaining activity
70
purified enzyme, loss of 75% activity within 10 min, 240 min: loss of 91% activity
70
-
presence of free SH-groups required
70
-
below, but tolerates exposure up to a few hours
70
-
several hours, stable
80
-
20 min, purified recombinant enzyme, stable
80
20 min, purified recombinant enzyme, stable
additional information
thermal inactivation through transition of the hexameric to the monomeric form
additional information
-
the enzyme is heat-stable
additional information
-
the enzyme is heat-stable