Literature summary for 3.4.11.10 extracted from

Bzymek, K.P.; D'Souza, V.M.; Chen, G.; Campbell, H.; Mitchell, A.; Holz, R.C.
Function of the signal peptide and N- and C-terminal propeptides in the leucine aminopeptidase from Aeromonas proteolytica (2004), Protein Expr. Purif., 37, 294-305.

Search for more literature for 3.4.11.10

Cloned(Commentary)

Cloned (Comment)	Organism
gene AAP, overexpression of different forms of AAP in Escherichia coli, i.e as MBP-fusion protein, with or without N- and/or C-terminal propeptides, or with the native leader sequence of the enzyme	Vibrio proteolyticus

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the propeptides internally inhibits the enzyme in the 54 kDa zymogen in a cooperative inhibitory interaction	Vibrio proteolyticus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics of different native and recombinant enzyme forms, overview	Vibrio proteolyticus
0.0106	-	L-Leu-4-nitroanilide	pH 8.0, 25°C, mature wild-type enzyme	Vibrio proteolyticus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	the enzyme contains a signal peptide sequence for protein secretion	Vibrio proteolyticus	-	-
periplasm	-	Vibrio proteolyticus	-	-
soluble	-	Vibrio proteolyticus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	AAP is a metalloenzyme containing two Zn2+ per enzyme molecule	Vibrio proteolyticus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
32000	-	processed enzyme form	Vibrio proteolyticus
43000	-	unprocessed enzyme form	Vibrio proteolyticus

Organism

Organism	UniProt	Comment	Textmining
Vibrio proteolyticus	Q01693	i.e. Vibrio proteolyticus	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	the enzyme contains N- and C-terminal propeptides, processing of a 54 kDa zymogen to the 32 kDa mature enzyme, overview, modeling of the processing of wild-type enzyme, mechanism	Vibrio proteolyticus

Purification (Commentary)

Purification (Comment)	Organism
native periplasmic enzyme by ion exchange and hydrophobic interaction chromatography, recombinant MBP-fusion enzyme and recombinant extracellular or mature intracellular wild-type enzyme from Escherichia coli by amylose affinity chromatography and ultrafiltration, and by ammonium sulfate fractionation, dialysis, hydrophobic interaction and anion exchange chromatography, and a second hydrophobic interaction chromatography step	Vibrio proteolyticus

Purification (Comment)

Organism

native periplasmic enzyme by ion exchange and hydrophobic interaction chromatography, recombinant MBP-fusion enzyme and recombinant extracellular or mature intracellular wild-type enzyme from Escherichia coli by amylose affinity chromatography and ultrafiltration, and by ammonium sulfate fractionation, dialysis, hydrophobic interaction and anion exchange chromatography, and a second hydrophobic interaction chromatography step

Vibrio proteolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Vibrio proteolyticus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Leu-4-nitroanilide + H2O	-	Vibrio proteolyticus	L-Leu + 4-nitroaniline	-	?
additional information	the enzyme shows broad substrate specificity	Vibrio proteolyticus	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 44000-46300, unprocessed periplasmic enzyme, SDS-PAGE, x * 31000-32000, processed extracellular and periplasmic enzyme, SDS-PAGE, x * 39000-40000, unprocessed extracellular enzyme, SDS-PAGE,x * 51000-52000, unprocessed recombinant enzyme, SDS-PAGE	Vibrio proteolyticus

Synonyms

Synonyms	Comment	Organism
AAP	-	Vibrio proteolyticus
leucine aminopeptidase	-	Vibrio proteolyticus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Vibrio proteolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.6	-	L-Leu-4-nitroanilide	pH 8.0, 25°C, mature wild-type enzyme	Vibrio proteolyticus
71.33	-	L-Leu-4-nitroanilide	pH 8.0, 25°C, mature wild-type enzyme	Vibrio proteolyticus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Vibrio proteolyticus