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3.4.11.10: bacterial leucyl aminopeptidase

This is an abbreviated version!
For detailed information about bacterial leucyl aminopeptidase, go to the full flat file.

Word Map on EC 3.4.11.10

Reaction

release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids =

Synonyms

AAP, Aeromonas proteolytica aminopeptidase, Aminopeptidase, aminopeptidase A, aminopeptidase A (bacteria), aminopeptidase Ap1, aminopeptidase II, AP-II, API, APII, AVP, bacterial leucine aminopeptidase, bacterial M17 aminopeptidase, BSAP, Bsu aminopeptidase, BsuAP, CGase, cysteinylglycinase, double-zinc aminopeptidase, extracellular aminopeptidase, FgLAP, HpM17AP, LAP, LAPII, leucine aminopeptidase, leucine aminopeptidase II, leucine APN, Leucyl aminopeptidase, M17 aminopeptidase, M17 metallo-aminopeptidase, More, MtLAP, PepA, Peptidase A, pepZ, PhpA, ribosomal-bound aminopeptidase, rLAP55, Rv2213, SSAP, TAP, TH-2, thermophilic aminopeptidase, thermostable leucine aminopeptidase, Vibrio aminopeptidase, VpAP, ywaD

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.10 bacterial leucyl aminopeptidase

Cloned

Cloned on EC 3.4.11.10 - bacterial leucyl aminopeptidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis
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DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain AD494(DE3)
expressed in Escherichia coli
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expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli BL21 Star DE3 cells
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21-Star(DE3) cells
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expressed in Escherichia coli M15 cells
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expressed in Escherichia coli TOP 10 cells
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expressed in Pichia pastoris
-
expression in Escherichia coli
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain M15
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expression of the chimeric enzyme comprising the raw-starch-binding domain of Bacillus sp. strain TS-23 alpha-amylase anthe Bacillus stearothermophilus LAPII in Escherichia coli strain M15
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expression of wild-type and modified Lys-tagged enzymes in Escherichia coli strain M15
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expression of wild-type and mutant E151H enzymes in Escherichia coli strain BL21(DE3)
expression of wild-type and mutant E151HA and E151D enzymes in Escherichia coli strain BL21(DE3)
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gene AAP, overexpression of different forms of AAP in Escherichia coli, i.e as MBP-fusion protein, with or without N- and/or C-terminal propeptides, or with the native leader sequence of the enzyme
gene lap from genomic library, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli
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gene LAP, DNA and amino acid sequence determination and analysis, functional expression of His-tagged enzyme in Escherichia coli
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gene pepA, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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gene pepA, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL10-gold
gene ywad, overexpression in Escherichia coli
His-tagged protein expressed in Escherichia coli MM294
overexpression
-
overexpression in Escherichia coli
recombinant expression in Pichia pastoris strain GS115, the enzyme is secreted, the recombinant enzyme level is 7.1 times that of wild-type Bacillus subtilis strain Zj016, subcloning in Escherichia coli strain JM109
recombinant expression of His6-tagged enzyme
sequence comparisons, overexpression of His-tagged wild-type and mutant LAPII in Escherichia coli