Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.11.10 extracted from

  • Hwang, G.Y.; Kuo, L.Y.; Tsai, M.R.; Yang, S.L.; Lin, L.L.
    Histidines 345 and 378 of Bacillus stearothermophilus leucine aminopeptidase II are essential for the catalytic activity of the enzyme (2005), Antonie van Leeuwenhoek, 87, 355-359.
    View publication on PubMed
Search for more literature for 3.4.11.10

Cloned(Commentary)

Cloned (Comment) Organism
sequence comparisons, overexpression of His-tagged wild-type and mutant LAPII in Escherichia coli Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
H191L site-directed mutagenesis, the mutant enzyme shows similar catalytic effciency compared to the wild-type enzyme, with 30% increased Km Geobacillus stearothermophilus
H227L site-directed mutagenesis, the mutant enzyme shows a 39% decreased catalytic effciency compared to the wild-type enzyme, with a 3.9fold increased Km Geobacillus stearothermophilus
H345L site-directed mutagenesis, inactive mutant enzyme Geobacillus stearothermophilus
H378L site-directed mutagenesis, inactive mutant enzyme Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
 L-Leu-4-nitroanilide 60°C, recombinant wild-type enzyme Geobacillus stearothermophilus
1.2
-
 L-Leu-4-nitroanilide 60°C, recombinant mutant H191L Geobacillus stearothermophilus
4.4
-
 L-Leu-4-nitroanilide 60°C, recombinant mutant H227L Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44500
-
 x * 44500, recombinant His-tagged wild-type and mutant LAPII, SDS-PAGE Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus P24828
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant LAPII from Escherichia coli by nickel chelate affinity chromatography Geobacillus stearothermophilus

Reaction

Reaction Comment Organism Reaction ID
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids. residues His345 and His378 play an important role in catalysis Geobacillus stearothermophilus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
60.8
-
 purified recombinant mutant H227L Geobacillus stearothermophilus
91.3
-
 purified recombinant mutant H191L Geobacillus stearothermophilus
133.4
-
 purified recombinant wild-type enzyme Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu-4-nitroanilide + H2O
-
 Geobacillus stearothermophilus L-Leu + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 44500, recombinant His-tagged wild-type and mutant LAPII, SDS-PAGE Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
aminopeptidase II
-
 Geobacillus stearothermophilus
AP-II
-
 Geobacillus stearothermophilus
LAPII
-
 Geobacillus stearothermophilus
leucine aminopeptidase II
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Geobacillus stearothermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
 L-Leu-4-nitroanilide 60°C, recombinant wild-type enzyme Geobacillus stearothermophilus
0.46
-
 L-Leu-4-nitroanilide 60°C, recombinant mutant H191L Geobacillus stearothermophilus
0.86
-
 L-Leu-4-nitroanilide 60°C, recombinant mutant H227L Geobacillus stearothermophilus