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Literature summary for 3.4.11.10 extracted from

  • Bienvenue, D.L.; Gilner, D.; Holz, R.C.
    Hydrolysis of thionopeptides by the aminopeptidase from Aeromonas proteolytica: insight into substrate binding (2002), Biochemistry, 41, 3712-3719.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1-Phenyl-2-thiourea competitive Vibrio proteolyticus
EDTA
-
Vibrio proteolyticus
L-leucine anilide
-
Vibrio proteolyticus
L-leucine anisidide
-
Vibrio proteolyticus
Phenylurea
-
Vibrio proteolyticus
thionoleucine-S-anilide
-
Vibrio proteolyticus
thionoleucine-S-anisidide
-
Vibrio proteolyticus
Urea competitive Vibrio proteolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics with Zn and/or Cd-containing enzyme, overview Vibrio proteolyticus
4
-
thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Cd2+ bound Vibrio proteolyticus
4
-
thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
4
-
thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
4.6
-
L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
5
-
thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
7
-
thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
7.8
-
L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
8
-
thionoleucine-S-anilide pH 8.0, 25°C, enzyme with 2 Cd2+ bound Vibrio proteolyticus
10
-
thionoleucine-S-anilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
11
-
thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
15
-
L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
32
-
L-leucine-4-anisidide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
34
-
L-leucine-4-anisidide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
45
-
L-leucine anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
50
-
L-leucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
61
-
L-leucine anilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
78
-
L-leucine anilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ the bimetallic enzyme contains either 2 Zn2+ or 2 Cd2+ or Zn2+ and Cd2+ Vibrio proteolyticus
Cd2+ can substitute for one or both of the Zn2+ ions Vibrio proteolyticus
Co2+ Co2+ can substitute for Cd2+ in the bimetallic enzyme Vibrio proteolyticus
Co2+ can substitute for one or both of the Zn2+ ions Vibrio proteolyticus
additional information bimetallic enzyme, binding conformations, overview Vibrio proteolyticus
Zn2+ the bimetallic enzyme contains either 2 Zn2+ or 2 Cd2+ or Zn2+ and Cd2+ Vibrio proteolyticus
Zn2+ zinc-metallopeptidase, 2 ions per enzyme molecule Vibrio proteolyticus

Organism

Organism UniProt Comment Textmining
Vibrio proteolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Vibrio proteolyticus
native enzyme Vibrio proteolyticus

Reaction

Reaction Comment Organism Reaction ID
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids. substrate binding mechanism and structure Vibrio proteolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu-4-nitroanilide + H2O
-
Vibrio proteolyticus L-Leu + 4-nitroaniline
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
-
Vibrio proteolyticus L-Leu + 7-amino-4-methylcoumarin
-
?
L-leucine 4-nitroanilide + H2O
-
Vibrio proteolyticus L-leucine + 4-nitroaniline
-
?
L-leucine anilide + H2O
-
Vibrio proteolyticus L-leucine + aniline
-
?
L-leucine-4-anisidide + H2O
-
Vibrio proteolyticus L-leucine + anisidine
-
?
thionoleucine-4-anisidide + H2O
-
Vibrio proteolyticus thionoleucine + anisidine
-
?
thionoleucine-S-anilide + H2O
-
Vibrio proteolyticus thionoleucine + aniline
-
?

Synonyms

Synonyms Comment Organism
AAP
-
Vibrio proteolyticus
Aeromonas proteolytica aminopeptidase
-
Vibrio proteolyticus
More see also EC 3.4.11.22 Vibrio proteolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Vibrio proteolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.8
-
L-leucine-4-anisidide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
5.3
-
L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
6.3
-
L-leucine anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
7.5
-
thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
7.7
-
thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
7.9
-
thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Cd2+ bound Vibrio proteolyticus
9.3
-
L-leucine-4-anisidide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
9.5
-
L-leucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
10.6
-
thionoleucine-S-anilide pH 8.0, 25°C, enzyme with 2 Cd2+ bound Vibrio proteolyticus
11.1
-
thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
11.5
-
thionoleucine-S-anilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
12.2
-
thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
15.1
-
L-leucine anilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
16
-
L-leucine anilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
16.9
-
thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
23.3
-
thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
60
-
L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Vibrio proteolyticus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics with Zn and/or Cd-containing enzyme, overview Vibrio proteolyticus
0.24
-
1-Phenyl-2-thiourea pH 8.0, 25°C Vibrio proteolyticus
4.6
-
Urea pH 8.0, 25°C Vibrio proteolyticus