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Literature summary for 3.4.11.10 extracted from
- Characterization of an N-glycosylated Bacillus subtilis leucine aminopeptidase expressed in Pichia pastoris (2015), J. Basic Microbiol., 55, 236-246.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DL-dithiothreitol | - |
Bacillus subtilis |  |
| EDTA | - |
Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.97 | - |
leucine-4-nitroaniline | pH 8.5, 60°C | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibits activity | Bacillus subtilis | |
| Ca2+ | weakly inhibits activity | Bacillus subtilis | |
| Co2+ | enhances activity | Bacillus subtilis | |
| Cu2+ | weakly inhibits activity | Bacillus subtilis | |
| Ni2+ | inhibits activity | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | enzyme contains three potential glycosylation sites | Bacillus subtilis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using ion-exchange chromatography, ultrafiltration and gel filtration | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| leucine-4-nitroaniline + H2O | enzyme also shows activity towards Arg-4-nitroaniline, Lys-4-nitroaniline and Met-4-nitroaniline | Bacillus subtilis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| leucine aminopeptidase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
recombinant protein | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | recombinant protein | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
recombinant protein | Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | recombinant protein | Bacillus subtilis |
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Release 2023.1 (January 2023)
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