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3.4.11.10: bacterial leucyl aminopeptidase

This is an abbreviated version!
For detailed information about bacterial leucyl aminopeptidase, go to the full flat file.

Word Map on EC 3.4.11.10

Reaction

release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids =

Synonyms

AAP, Aeromonas proteolytica aminopeptidase, Aminopeptidase, aminopeptidase A, aminopeptidase A (bacteria), aminopeptidase Ap1, aminopeptidase II, AP-II, API, APII, AVP, bacterial leucine aminopeptidase, bacterial M17 aminopeptidase, BSAP, Bsu aminopeptidase, BsuAP, CGase, cysteinylglycinase, double-zinc aminopeptidase, extracellular aminopeptidase, FgLAP, HpM17AP, LAP, LAPII, leucine aminopeptidase, leucine aminopeptidase II, leucine APN, Leucyl aminopeptidase, M17 aminopeptidase, M17 metallo-aminopeptidase, More, MtLAP, PepA, Peptidase A, pepZ, PhpA, ribosomal-bound aminopeptidase, rLAP55, Rv2213, SSAP, TAP, TH-2, thermophilic aminopeptidase, thermostable leucine aminopeptidase, Vibrio aminopeptidase, VpAP, ywaD

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.10 bacterial leucyl aminopeptidase

Specific Activity

Specific Activity on EC 3.4.11.10 - bacterial leucyl aminopeptidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0001
-
recombinant enzyme, using L-Glu-7-amido-4-methylcoumarin as substrate, at 37°C
0.0002
-
native enzyme, using L-Glu-7-amido-4-methylcoumarin as substrate, at 37°C
0.0003
-
native enzyme, using L-Asp-7-amido-4-methylcoumarin as substrate, at 37°C
0.0005
-
native enzyme, using Gly-7-amido-4-methylcoumarin as substrate, at 37°C
0.0006
-
native enzyme, using L-Val-7-amido-4-methylcoumarin as substrate, at 37°C
0.001
purified enzyme
0.0012
-
recombinant enzyme, using L-Asp-7-amido-4-methylcoumarin as substrate, at 37°C
0.0019
0.0021
-
recombinant enzyme, using L-Ser-7-amido-4-methylcoumarin as substrate, at 37°C
0.0025
-
recombinant enzyme, using L-Val-7-amido-4-methylcoumarin as substrate, at 37°C
0.003
supernatant clarification, in 10 mM Tricine buffer, pH 8.0, at 25°C
0.0032
-
native enzyme, using L-Pro-7-amido-4-methylcoumarin as substrate, at 37°C
0.0054
-
native enzyme, using L-Tyr-7-amido-4-methylcoumarin as substrate, at 37°C
0.0056
-
recombinant enzyme, using L-Pro-7-amido-4-methylcoumarin as substrate, at 37°C
0.0062
-
recombinant enzyme, using L-Ile-7-amido-4-methylcoumarin as substrate, at 37°C
0.0064
-
recombinant enzyme, using L-Tyr-7-amido-4-methylcoumarin as substrate, at 37°C
0.0068
-
recombinant enzyme, using L-Phe-7-amido-4-methylcoumarin as substrate, at 37°C
0.0075
-
recombinant enzyme, using L-Ala-7-amido-4-methylcoumarin as substrate, at 37°C
0.0083
-
native enzyme, using L-Ala-7-amido-4-methylcoumarin as substrate, at 37°C
0.0093
-
native enzyme, using L-Ile-7-amido-4-methylcoumarin as substrate, at 37°C
0.0141
-
native enzyme, using L-Phe-7-amido-4-methylcoumarin as substrate, at 37°C
0.0253
-
recombinant enzyme, using L-Met-7-amido-4-methylcoumarin as substrate, at 37°C
0.0291
-
recombinant enzyme, using L-Arg-7-amido-4-methylcoumarin as substrate, at 37°C
0.0355
-
recombinant enzyme, using L-Thr-7-amido-4-methylcoumarin as substrate, at 37°C
0.0657
-
native enzyme, using L-Met-7-amido-4-methylcoumarin as substrate, at 37°C
0.0694
-
native enzyme, using L-Thr-7-amido-4-methylcoumarin as substrate, at 37°C
0.0752
-
native enzyme, using L-Arg-7-amido-4-methylcoumarin as substrate, at 37°C
0.0831
-
recombinant enzyme, using L-Cys-7-amido-4-methylcoumarin as substrate, at 37°C
0.0953
-
native enzyme, using L-Cys-7-amido-4-methylcoumarin as substrate, at 37°C
0.1344
-
native enzyme, using L-Leu-7-amido-4-methylcoumarin as substrate, at 37°C
0.2145
-
recombinant enzyme, using L-Leu-7-amido-4-methylcoumarin as substrate, at 37°C
10.41
-
free recombinant wild-type enzyme
104.9
-
mutant enzyme Y532D, at 60°C, using L-Leu-4-nitroanilide as substrate
13.8
-
mutant enzyme D380E, at 60°C, using L-Leu-4-nitroanilide as substrate
132.5
purified native enzyme, pH 8.5, 50°C, substrate Leu-4-nitroanilide
133.4
purified recombinant wild-type enzyme
15.1
-
mutant enzyme D380V, at 60°C, using L-Leu-4-nitroanilide as substrate
15.86
-
immobilized recombinant wild-type enzyme
16
substrate L-leucine-4-nitroanilidepH 8.0, 37°C
17
-
nitro-enzyme
18.22
-
free recombinant Lys9-tagged enzyme
20
-
1-ethyl-3(3-dimethylaminopropyl)-carbodiimide treated enzyme
21.71
-
free recombinant Lys3-tagged enzyme
22
-
mutant enzyme D198M/F221W, using L-Leu-p-nitroanilide as a substrate
240.48
purified recombinant enzyme, pH 8.5, 50°C, substrate Leu-4-nitroanilide
25
-
value about, substrate Gly-7-amido-4-methylcoumarin
3
-
azo-enzyme
34.5
-
mutant enzyme Y532L, at 60°C, using L-Leu-4-nitroanilide as substrate
4.17
-
value about, substrate L-Pro-7-amido-4-methylcoumarin
466.7
-
purified recombinant enzyme
55.3
-
mutant enzyme D380N, at 60°C, using L-Leu-4-nitroanilide as substrate
572.5
-
wild type enzyme, at 60°C, using L-Leu-4-nitroanilide as substrate
60.73
-
immobilized recombinant Lys3-tagged enzyme
60.8
purified recombinant mutant H227L
64
purified recombinant enzyme
68
-
wild type enzyme, using L-Leu-p-nitroanilide as a substrate
76.91
-
immobilized recombinant Lys9-tagged enzyme
8.3
-
value about, substrate L-Tyr-7-amido-4-methylcoumarin
80 - 115
-
native enzyme, buffer dependent
84
-
diethylpyrocarbonate-treated enzyme
91.3
purified recombinant mutant H191L
91.6
-
purified recombinant wild-type enzyme
additional information