3.4.11.10: bacterial leucyl aminopeptidase
This is an abbreviated version!
For detailed information about bacterial leucyl aminopeptidase, go to the full flat file.
Word Map on EC 3.4.11.10
Reaction
release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids =
Synonyms
AAP, Aeromonas proteolytica aminopeptidase, Aminopeptidase, aminopeptidase A, aminopeptidase A (bacteria), aminopeptidase Ap1, aminopeptidase II, AP-II, API, APII, AVP, bacterial leucine aminopeptidase, bacterial M17 aminopeptidase, BSAP, Bsu aminopeptidase, BsuAP, CGase, cysteinylglycinase, double-zinc aminopeptidase, extracellular aminopeptidase, FgLAP, HpM17AP, LAP, LAPII, leucine aminopeptidase, leucine aminopeptidase II, leucine APN, Leucyl aminopeptidase, M17 aminopeptidase, M17 metallo-aminopeptidase, More, MtLAP, PepA, Peptidase A, pepZ, PhpA, ribosomal-bound aminopeptidase, rLAP55, Rv2213, SSAP, TAP, TH-2, thermophilic aminopeptidase, thermostable leucine aminopeptidase, Vibrio aminopeptidase, VpAP, ywaD
ECTree
3.4.11.10 bacterial leucyl aminopeptidaseAdvanced search results
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results (KM Value
KM Value on EC 3.4.11.10 - bacterial leucyl aminopeptidase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.231
L-Ala-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1 mM MnCl2, at 37°C
0.078
L-Arg-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1 mM MnCl2, at 37°C
0.077
L-Cys-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1 mM MnCl2, at 37°C
0.066
L-Phe-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1 mM MnCl2, at 37°C
0.06
L-Thr-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1 mM MnCl2, at 37°C
0.02726
recombinant human methionine-interferon alpha-2b
pH 6.0, 37°C
-
7.51
L-Arg-p-nitroanilide
-
mutant enzyme D198P/F221E, at pH 8.0 and 37°C
0.056
L-cystinyl-p-nitroanilide
-
mutant enzyme D198I/F221W, at pH 8.0 and 37°C
0.01
L-Leu-4-nitroanilide
mutant enzyme D118N, in 50 mM Tricine buffer, 1.0 mM ZnSO4, and 200 mM KCl, at pH 8.0 and 25°C
0.0106
L-Leu-4-nitroanilide
pH 8.0, 25°C, mature wild-type enzyme
0.013
L-Leu-4-nitroanilide
wild type enzyme, in 50 mM Tricine buffer, 1.0 mM ZnSO4, and 200 mM KCl, at pH 8.0 and 25°C
0.025
L-Leu-4-nitroanilide
mutant enzyme S228A, in 50 mM Tricine buffer, 1.0 mM ZnSO4, and 200 mM KCl, at pH 8.0 and 25°C
0.0317
L-Leu-4-nitroanilide
apparent value, mutant enzyme D99M, in 10 mM Tricine buffer, pH 8.0, at 25°C
0.245
L-Leu-4-nitroanilide
pH 8.0, 37°C, recombinant His6-tagged enzyme, with preincubation (72 h of incubation at 37°C)
0.311
L-Leu-4-nitroanilide
pH 8.0, 70°C, recombinant His6-tagged enzyme, with preincubation (72 h of incubation at 37°C)
0.34
L-Leu-4-nitroanilide
mutant enzyme M180A, in 50 mM Tricine buffer, 1.0 mM ZnSO4, and 200 mM KCl, at pH 8.0 and 25°C
0.9
L-Leu-4-nitroanilide
60°C, recombinant wild-type enzyme
0.902
L-Leu-4-nitroanilide
pH 8.0, 37°C, recombinant His6-tagged enzyme, without preincubation
0.95
L-Leu-4-nitroanilide
-
wild type enzyme, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
1.2
L-Leu-4-nitroanilide
60°C, recombinant mutant H191L
2.1
L-Leu-4-nitroanilide
-
pH 8.0, 55°C, recombinant wild-type enzyme
2.12
L-Leu-4-nitroanilide
-
mutant enzyme Y352D, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
2.83
L-Leu-4-nitroanilide
-
mutant enzyme Y352L, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
3.05
L-Leu-4-nitroanilide
-
mutant enzyme D380N, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
3.39
L-Leu-4-nitroanilide
-
mutant enzyme D380E, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
3.41
L-Leu-4-nitroanilide
-
mutant enzyme D380V, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
4.4
L-Leu-4-nitroanilide
60°C, recombinant mutant H227L
0.038
L-Leu-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1 mM MnCl2, at 37°C
1.15
L-Leu-p-nitroanilide
-
mutant enzyme D198F/F221I, at pH 8.0 and 37°C
0.019 - 0.0215
L-leucine 4-nitroanilide
-
native enzyme, buffer dependent
0.055
L-leucine 4-nitroanilide
-
1-ethyl-3(3-dimethylaminopropyl)-carbodiimide treated enzyme
4.6
L-leucine 4-nitroanilide
-
pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound
7.8
L-leucine 4-nitroanilide
-
pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound
32
L-leucine-4-anisidide
-
pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound
34
L-leucine-4-anisidide
-
pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound
0.093
L-Met-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1 mM MnCl2, at 37°C
4
thionoleucine-4-anisidide
-
pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound
5
thionoleucine-4-anisidide
-
pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound
4
thionoleucine-S-anilide
-
pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound
11
thionoleucine-S-anilide
-
pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound
additional information
additional information
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kinetics and thermodynamics
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additional information
additional information
kinetics with metal-bound enzyme variants
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additional information
additional information
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kinetics of apoenzyme and metal-bound wild-type and mutant enzymes, isothermal titration measurement, thermodynamics, overview
-
additional information
additional information
kinetics of different native and recombinant enzyme forms, overview
-
additional information
additional information
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kinetics of raw starch-binding by the chimeric mutant enzyme
-
additional information
additional information
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kinetics of wild-type and methionine mutant enzymes, overview
-
additional information
additional information
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kinetics with Zn and/or Cd-containing enzyme, overview
-
additional information
additional information
pH-dependence of kinetics, isothermal titration measurement, thermodynamics, overview
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additional information
additional information
thermodynamics and Michaelis-Menten kinetics, overview
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