Cloned (Comment) | Organism |
---|---|
expression of the chimeric enzyme comprising the raw-starch-binding domain of Bacillus sp. strain TS-23 alpha-amylase anthe Bacillus stearothermophilus LAPII in Escherichia coli strain M15 | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a chimeric enzyme comprising the raw-starch-binding domain of Bacillus sp. strain TS-23 alpha-amylase and the Bacillus stearothermophilus LAPII, the chimeric mutant enzyme binds raw starch, overview | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of raw starch-binding by the chimeric mutant enzyme | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
x * 61000, recombinant chimeric enzyme, SDS-PAGE | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant chimeric enzyme 20.4fold from Escherichia coli strain M15 by an affinity adsorption chromatographic step on a raw starch resin, screening of different elution methods, overview | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
466.7 | - |
purified recombinant enzyme | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Leu-4-nitroanilide + H2O | - |
Geobacillus stearothermophilus | L-Leu + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61000, recombinant chimeric enzyme, SDS-PAGE | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
LAP | - |
Geobacillus stearothermophilus |
leucine aminopeptidase II | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Geobacillus stearothermophilus |