Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cysteine | 60% loss of activity at 10 mM | Vibrio proteolyticus | |
EDTA | - |
Vibrio proteolyticus | |
Na2S | 60% loss of activity at 10 mM | Vibrio proteolyticus | |
Thioglycollate | 60% loss of activity at 10 mM | Vibrio proteolyticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Vibrio proteolyticus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 65% of activity of zinc enzyme | Vibrio proteolyticus | |
Mn2+ | 58% of activity of zinc enzyme | Vibrio proteolyticus | |
Zn2+ | - |
Vibrio proteolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29500 | - |
sedimentation velocity | Vibrio proteolyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Peptides + H2O | Vibrio proteolyticus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio proteolyticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vibrio proteolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21 | - |
- |
Vibrio proteolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Gly + H2O | - |
Vibrio proteolyticus | Ala + Gly | - |
ir | |
DL-Ala-Gly-Gly + H2O | - |
Vibrio proteolyticus | DL-Ala + Gly-Gly | - |
ir | |
DL-Leu-Gly-DL-Phe + H2O | - |
Vibrio proteolyticus | DL-Leu + Gly-DL-Phe | - |
ir | |
DL-Leu-Gly-Gly + H2O | - |
Vibrio proteolyticus | DL-Leu + Gly-Gly | - |
ir | |
Leu-beta-naphthylamide + H2O | - |
Vibrio proteolyticus | Leu + beta-naphthylamine | - |
ir | |
Leu-Gly + H2O | - |
Vibrio proteolyticus | Leu + Gly | - |
ir | |
Leu-Tyr + H2O | - |
Vibrio proteolyticus | Leu + Tyr | - |
ir | |
Lys-Gly + H2O | - |
Vibrio proteolyticus | Lys + Gly | - |
ir | |
Peptides + H2O | - |
Vibrio proteolyticus | ? | - |
? | |
Phe-beta-naphthylamide + H2O | 18% of rate of hydrolysis of Leu-beta-naphthylamide | Vibrio proteolyticus | Phe + beta-naphthylamine | - |
ir | |
Phe-Gly + H2O | - |
Vibrio proteolyticus | Phe + Gly | - |
ir | |
Thr-beta-naphthylamide + H2O | 3.3% of rate of hydrolysis of Leu-beta-naphthylamide | Vibrio proteolyticus | Thr + beta-naphthylamine | - |
ir | |
Tyr-Gly + H2O | - |
Vibrio proteolyticus | Tyr + Gly | - |
ir | |
Val-Gly + H2O | - |
Vibrio proteolyticus | Val + Gly | - |
ir |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
below, but tolerates exposure up to a few hours | Vibrio proteolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Vibrio proteolyticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | above 40% maximal activity across range | Vibrio proteolyticus |