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Literature summary for 3.4.11.10 extracted from
- Aeromonas aminopeptidase (1976), Methods Enzymol., 45, 530-543.
General Stability
| General Stability | Organism |
|---|---|
| only partially inactivated in 8 M urea | Vibrio proteolyticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Vibrio proteolyticus |  |
| EDTA | - |
Vibrio proteolyticus | |
| Leu-bromomethyl ketone | succinamic acid derivative, Ki: 0.0069 mM | Vibrio proteolyticus | |
| Leu-methyl ketone | derivatives, Ki: 0.14 - 0.17 mM | Vibrio proteolyticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | amides and dipeptides | Vibrio proteolyticus | |
| 0.019 | - |
L-leucine 4-nitroanilide | - |
Vibrio proteolyticus | |
| 0.18 | - |
Leu-Leu | - |
Vibrio proteolyticus | |
| 0.35 | - |
Leu-Met | - |
Vibrio proteolyticus | |
| 0.38 | - |
Leu-Ile | - |
Vibrio proteolyticus | |
| 0.39 | - |
Leu-Arg | - |
Vibrio proteolyticus | |
| 0.43 | - |
Leu-beta-naphthylamide | - |
Vibrio proteolyticus | |
| 0.64 | - |
Leu-Val | - |
Vibrio proteolyticus | |
| 0.86 | - |
Leu-Phe | - |
Vibrio proteolyticus | |
| 0.96 | - |
Leu-Trp | - |
Vibrio proteolyticus | |
| 1 | - |
Leu-Ala | - |
Vibrio proteolyticus | |
| 1.5 | - |
Leu-Tyr | - |
Vibrio proteolyticus | |
| 3.9 | - |
Phe-methyl ester | - |
Vibrio proteolyticus | |
| 5.1 | - |
Leu-amide | - |
Vibrio proteolyticus | |
| 6.3 | - |
norleucinamide | - |
Vibrio proteolyticus | |
| 8.5 | - |
Leu-methyl ester | - |
Vibrio proteolyticus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Vibrio proteolyticus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Vibrio proteolyticus | |
| Mn2+ | - |
Vibrio proteolyticus | |
| Zn2+ | - |
Vibrio proteolyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28480 | - |
sedimentation equilibrium | Vibrio proteolyticus |
| 29500 | - |
sedimentation velocity | Vibrio proteolyticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Peptides + H2O | Vibrio proteolyticus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio proteolyticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vibrio proteolyticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 35.5 | - |
- |
Vibrio proteolyticus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10 M tricine buffer, pH 8.0-8.5, several years, little loss of activity | Vibrio proteolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Leu-4-nitroanilide + H2O | - |
Vibrio proteolyticus | Leu + 4-nitroaniline | - |
ir | |
| Leu-Ala + H2O | - |
Vibrio proteolyticus | Leu + Ala | - |
ir | |
| Leu-amide + H2O | - |
Vibrio proteolyticus | Leu + NH3 | - |
ir | |
| Leu-Arg + H2O | - |
Vibrio proteolyticus | Leu + Arg | - |
ir | |
| Leu-beta-naphthylamide + H2O | - |
Vibrio proteolyticus | Leu + beta-naphthylamine | - |
ir | |
| Leu-Ile + H2O | - |
Vibrio proteolyticus | Leu + Ile | - |
ir | |
| Leu-Leu + H2O | - |
Vibrio proteolyticus | Leu + Leu | - |
ir | |
| Leu-Met + H2O | - |
Vibrio proteolyticus | Leu + Met | - |
ir | |
| Leu-methyl ester + H2O | - |
Vibrio proteolyticus | Leu + methanol | - |
ir | |
| Leu-Phe + H2O | - |
Vibrio proteolyticus | Leu + Phe | - |
ir | |
| Leu-Trp + H2O | - |
Vibrio proteolyticus | Leu + Trp | - |
ir | |
| Leu-Tyr + H2O | - |
Vibrio proteolyticus | Leu + Tyr | - |
ir | |
| Leu-Val + H2O | - |
Vibrio proteolyticus | Leu + Val | - |
ir | |
| additional information | also cleaved: gastrin peptide (carboxyterminal), various oligopeptides, Met-Lys-bradykinin, adrenocorticotropic hormone, glucagon, aminoethylated B-chain insulin, sperm whale myoglobin, ribunuclease, beta-lactoglobulin, egg white lysozyme, aminoethylated lysozyme | Vibrio proteolyticus | ? | - |
? | |
| norleucinamide + H2O | - |
Vibrio proteolyticus | norleucine + NH3 | - |
ir | |
| Peptides + H2O | - |
Vibrio proteolyticus | ? | - |
? | |
| Phe-Asp-Ser-Ala-Val + H2O | - |
Vibrio proteolyticus | Phe + Asp-Ser-Ala-Val | - |
ir | |
| Phe-methyl ester + H2O | - |
Vibrio proteolyticus | Phe + methanol | - |
ir | |
| Pro-Phe-Gly-Lys + H2O | - |
Vibrio proteolyticus | Pro + Phe-Gly-Lys | - |
ir | |
| Pro-Phe-Pro + H2O | - |
Vibrio proteolyticus | Pro + Phe-Pro | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | SDS-PAGE | Vibrio proteolyticus |
| More | amino acid composition | Vibrio proteolyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Vibrio proteolyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
below, but tolerates exposure up to a few hours | Vibrio proteolyticus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.3 | - |
Leu-Leu | - |
Vibrio proteolyticus | |
| 15 | - |
Leu-Ala | - |
Vibrio proteolyticus | |
| 15 | - |
Leu-Val | - |
Vibrio proteolyticus | |
| 16 | - |
Leu-Ile | - |
Vibrio proteolyticus | |
| 29.6 | - |
Leu-beta-naphthylamide | - |
Vibrio proteolyticus | |
| 39 | - |
Leu-Arg | - |
Vibrio proteolyticus | |
| 42 | - |
Phe-methyl ester | - |
Vibrio proteolyticus | |
| 53 | - |
Leu-Met | - |
Vibrio proteolyticus | |
| 58 | - |
Leu-Trp | - |
Vibrio proteolyticus | |
| 60 | - |
norleucinamide | - |
Vibrio proteolyticus | |
| 67 | - |
L-leucine 4-nitroanilide | - |
Vibrio proteolyticus | |
| 72 | - |
Leu-Phe | - |
Vibrio proteolyticus | |
| 85 | - |
Leu-Tyr | - |
Vibrio proteolyticus | |
| 145 | - |
Leu-methyl ester | - |
Vibrio proteolyticus | |
| 220 | - |
Leu-amide | - |
Vibrio proteolyticus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Vibrio proteolyticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Vibrio proteolyticus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
Leu-bromomethyl ketone | - |
Vibrio proteolyticus | |
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