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Literature summary for 3.4.11.10 extracted from
- Purification and characterization of hyperthermotolerant leucine aminopeptidase from Geobacillus thermoleovorans 47b (2005), J. Ind. Microbiol. Biotechnol., 32, 269-276.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Geobacillus thermoleovorans |  |
| 2-mercaptoethanol | - |
Geobacillus thermoleovorans | |
| bestatin | - |
Geobacillus thermoleovorans | |
| DTT | - |
Geobacillus thermoleovorans | |
| EDTA | - |
Geobacillus thermoleovorans | |
| iodoacetate | - |
Geobacillus thermoleovorans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.658 | - |
L-Leu-4-nitroanilide | pH 7.6-7.8, 60°C | Geobacillus thermoleovorans | |
| 25 | - |
L-Leu-Gly-Gly | pH 7.6-7.8, 60°C | Geobacillus thermoleovorans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | reactivates the apoenzyme | Geobacillus thermoleovorans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42977 | - |
1 * 42977, mass spectrometry | Geobacillus thermoleovorans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus thermoleovorans | - |
- |
- |
| Geobacillus thermoleovorans 47b | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 164fold to homogeneity by ammonium sulfate fractionation, ion exchange and hydroxyapatite chromatography, again ion exchange chromatography, and gel filtration, followed by again ion exchange and hydroxyapatite chromatography | Geobacillus thermoleovorans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Geobacillus thermoleovorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Arg-4-nitroanilide + H2O | - |
Geobacillus thermoleovorans | L-Arg + 4-nitroaniline | - |
? | |
| L-Arg-4-nitroanilide + H2O | - |
Geobacillus thermoleovorans 47b | L-Arg + 4-nitroaniline | - |
? | |
| L-Leu-4-nitroanilide + H2O | - |
Geobacillus thermoleovorans | L-Leu + 4-nitroaniline | - |
? | |
| L-Leu-4-nitroanilide + H2O | - |
Geobacillus thermoleovorans 47b | L-Leu + 4-nitroaniline | - |
? | |
| L-Leu-Gly-Gly + H2O | - |
Geobacillus thermoleovorans | L-Leu + Gly-Gly | - |
? | |
| L-Leu-Gly-Gly + H2O | - |
Geobacillus thermoleovorans 47b | L-Leu + Gly-Gly | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42977, mass spectrometry | Geobacillus thermoleovorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LAP | - |
Geobacillus thermoleovorans |
| leucine aminopeptidase | - |
Geobacillus thermoleovorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Geobacillus thermoleovorans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
1 h, stable up to | Geobacillus thermoleovorans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10180 | - |
L-Leu-4-nitroanilide | pH 7.6-7.8, 60°C | Geobacillus thermoleovorans | |
| 49540 | - |
L-Leu-Gly-Gly | pH 7.6-7.8, 60°C | Geobacillus thermoleovorans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 7.8 | - |
Geobacillus thermoleovorans |
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