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Literature summary for 3.4.11.10 extracted from

  • Fundoiano-Hershcovitz, Y.; Rabinovitch, L.; Shulami, S.; Reiland, V.; Shoham, G.; Shoham, Y.
    The ywad gene from Bacillus subtilis encodes a double-zinc aminopeptidase (2005), FEMS Microbiol. Lett., 243, 157-163.
    View publication on PubMed
Search for more literature for 3.4.11.10

Cloned(Commentary)

Cloned (Comment) Organism
gene ywad, overexpression in Escherichia coli Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline complete inhibition, reversible by Zn2+ Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Bacillus subtilis
0.6
-
 L-Arg-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
0.85
-
 L-Lys-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
3.6
-
 L-Leu-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
10
-
 L-Ala-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
19
-
 L-Val-4-nitroanilide pH 8.0, 30°C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc-metalloaminopeptidase, 2 Zn2+ per enzyme molecule Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P25152 ywad gene
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 5fold from Escherichia coli by ion exchange chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus subtilis
64
-
 purified recombinant enzyme Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-4-nitroanilide + H2O low activity Bacillus subtilis L-Ala + 4-nitroaniline
-
 ?
L-Arg-4-nitroanilide + H2O best substrate Bacillus subtilis L-Arg + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Bacillus subtilis L-Leu + 4-nitroaniline
-
 ?
L-Lys-4-nitroanilide + H2O
-
 Bacillus subtilis L-Lys + 4-nitroaniline
-
 ?
L-Val-4-nitroanilide + H2O low activity Bacillus subtilis L-Val + 4-nitroaniline
-
 ?
additional information substrate specificity, 4-nitroanilides of Gly, Glu, Asp, Phe, Pro, and Ala-Pro are poor substrates, overview Bacillus subtilis ?
-
 ?

Synonyms

Synonyms Comment Organism
BSAP
-
 Bacillus subtilis
double-zinc aminopeptidase
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 20 min, purified recombinant enzyme, stable Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.37
-
 L-Ala-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
0.43
-
 L-Val-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
10
-
 L-Lys-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
34
-
 L-Arg-4-nitroanilide pH 8.0, 30°C Bacillus subtilis
55
-
 L-Leu-4-nitroanilide pH 8.0, 30°C Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
-
 Bacillus subtilis