Literature summary for 3.4.11.10 extracted from

Huynh, K.H.; Natarajan, S.; Choi, J.; Song, N.H.; Kim, J.G.; Lee, B.M.; Ahn, Y.J.; Kang, L.W.
Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of leucine aminopeptidase (LAP) from the pepA gene of Xanthomonas oryzae pv. oryzae (2009), Acta Crystallogr. Sect. F, 65, 952-955.

Search for more literature for 3.4.11.10

Cloned(Commentary)

Cloned (Comment)	Organism
gene pepA, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)	Xanthomonas oryzae pv. oryzae KACC 10331

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged enzyme, sitting drop vapour diffusion method, 10 mg/ml protein in 25 mM Tris-HCl, pH 7.5, 1 M NaCl, 3 mM 2-mercaptoethanol, 40 mM imidazole and 20% v/v glycerol, is mixed with precipitant solution containing 0.02 M CaCl2, 0.1 M sodium acetate, pH 4.6-5.0, and 15%, 20% or 40% v/v MPD, different conditions resulting in different crystal forms, X-ray diffraction structure determination and analysis at 2.6 A resolution	Xanthomonas oryzae pv. oryzae KACC 10331

Crystallization (Comment)

Organism

purified recombinant His-tagged enzyme, sitting drop vapour diffusion method, 10 mg/ml protein in 25 mM Tris-HCl, pH 7.5, 1 M NaCl, 3 mM 2-mercaptoethanol, 40 mM imidazole and 20% v/v glycerol, is mixed with precipitant solution containing 0.02 M CaCl2, 0.1 M sodium acetate, pH 4.6-5.0, and 15%, 20% or 40% v/v MPD, different conditions resulting in different crystal forms, X-ray diffraction structure determination and analysis at 2.6 A resolution

Xanthomonas oryzae pv. oryzae KACC 10331

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
51000	-	x * 51000, recombinant His-tagged enzyme, SDS-PAGE	Xanthomonas oryzae pv. oryzae KACC 10331

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Xanthomonas oryzae pv. oryzae KACC 10331	LAP is an exopeptidase that catalyzes the hydrolysis of leucine residues from the N-terminus of a protein or peptide	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Xanthomonas oryzae pv. oryzae KACC 10331	-	gene pepA or Xoo0834	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Xanthomonas oryzae pv. oryzae KACC 10331

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	LAP is an exopeptidase that catalyzes the hydrolysis of leucine residues from the N-terminus of a protein or peptide	Xanthomonas oryzae pv. oryzae KACC 10331	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 51000, recombinant His-tagged enzyme, SDS-PAGE	Xanthomonas oryzae pv. oryzae KACC 10331

Synonyms

Synonyms	Comment	Organism
LAP	-	Xanthomonas oryzae pv. oryzae KACC 10331
leucine aminopeptidase	-	Xanthomonas oryzae pv. oryzae KACC 10331