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3.1.4.37: 2',3'-cyclic-nucleotide 3'-phosphodiesterase

This is an abbreviated version!
For detailed information about 2',3'-cyclic-nucleotide 3'-phosphodiesterase, go to the full flat file.

Word Map on EC 3.1.4.37

Reaction

nucleoside 2',3'-cyclic phosphate
+
H2O
=
Nucleoside 2'-phosphate

Synonyms

2',3'-cyclic AMP phosphodiesterase, 2',3'-cyclic nucleoside monophosphate phosphodiesterase, 2',3'-cyclic nucleotide 3'-phosphodiesterase, 2',3'-cyclic nucleotide 3'-phosphohydrolase, 2',3'-cyclic nucleotide phosphodiesterase, 2',3'-cyclic nucleotide phosphohydrolase, 2',3'-cyclic nucleotide-3'-phosphodiesterase, 2',3'-cyclic nucleotide-3'-phosphohydrolase, 2',3'-cyclic phosphodiesterase, 2',3'-cyclic-nucleotide 3'-phosphodiesterase type I, 2':3'-CNMP-3'-ase, 2':3'-cyclic nucleotide 3'-phosphodiesterase, 3DMA, CNP, CNP1, CNP2, CNPase, CNPase I, cyclic 2',3'-nucleotide 3'-phosphodiesterase, cyclic 2',3'-nucleotide phosphodiesterase, cyclic-CMP phosphodiesterase, DGC2, nucleoside-2':3'-cyclic-phosphate 2'-nucleotidohydrolase, PaAcpH, phosphodiesterase, cyclic 2',3'-nucleotide 3'-, PNKP, RocR, YtqI, YybT

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
                3.1.4.37 2',3'-cyclic-nucleotide 3'-phosphodiesterase

Purification

Purification on EC 3.1.4.37 - 2',3'-cyclic-nucleotide 3'-phosphodiesterase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
10000fold
-
16000fold to near homogeneity
-
1990fold
-
by Ni-affinity chromatography followed by gel filtration
-
immunopurification, to homogeneity
-
Ni-NTA column chromatography and gel filtration
partial, 1196.4fold band 1, 739.9fold band 2, microsomes
-
partial, by delipidation, solubilization, gel chromatography, resolubilization, ion-exchange chromatography and affinity chromatography
-
recombinant CNP1 and C-terminal deletion mutant
-
recombinant His-tagged wild-type full-length enzyme and truncated mutants by nickel affinity chromatography and gel filtration or by calmodulin affinity chromatography
-
recombinant tRNA nucleotidyltransferase, EC 2.7.7.25, with its HD domain possessing 2',3'-cyclic phosphodiesterase activity
recombinant wild-type and mutant CNP1
-