Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect on enzyme activity by calmodulin binding | Mus musculus | |
additional information | no effect on enzyme activity by calmodulin binding | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged wild-type full-length enzyme and truncated mutants | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of truncated enzyme mutants: CNP_20-398, CNP_25-398, CNP_20-420, CNP_20-185, and CNP_179-398, analysis of calmodulin binding activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect on enzyme activity by calmodulin binding | Mus musculus | |
additional information | no effect on enzyme activity by calmodulin binding | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mus musculus | 16020 | - |
membrane | - |
Rattus norvegicus | 16020 | - |
additional information | the enzyme binds to calmodulin in a Ca2+-dependent manner via its binding site in the N-terminal domain, colocalization of CNPase and CaM during Schwann cell myelination in culture, molecular modeling | Rattus norvegicus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
isoform 2 | - |
Rattus norvegicus | - |
isoform 2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | two phosphorylation sites on Tyr110 and Ser169 within the N-terminal domain | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type full-length enzyme and truncated mutants by nickel affinity chromatography and gel filtration or by calmodulin affinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
CG-4 cell | - |
Rattus norvegicus | - |
dorsal root ganglion | DRG explant cultures | Mus musculus | - |
myelin membrane | the enzyme is a quantitatively major enzyme in myelin, where it localizes to the non-compact regions and is bound to the membrane surface | Rattus norvegicus | - |
Schwann cell | myelinating, colocalization of CNPase and CaM during Schwann cell myelination in culture | Rattus norvegicus | - |
Synonyms | Comment | Organism |
---|---|---|
2',3'-cyclic nucleotide 3'-phosphodiesterase | - |
Mus musculus |
2',3'-cyclic nucleotide 3'-phosphodiesterase | - |
Rattus norvegicus |
CNPase | - |
Mus musculus |
CNPase | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
additional information | role for the N-terminal domain of the enzyme in mediating multiple molecular interactions, molecular modeling, overview | Mus musculus |
additional information | role for the N-terminal domain of the enzyme in mediating multiple molecular interactions, molecular modeling, overview | Rattus norvegicus |
physiological function | the enzyme interacts with membrane surfaces, cytoskeletal proteins, and calmodulin | Rattus norvegicus |