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Literature summary for 3.1.4.37 extracted from
- Spectrophotometric assay, solubilization and purification of brain 2':3'-cyclic nucleotide 3-phosphodiesterase (1980), Biochem. J., 191, 71-82.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| hexadecyltrimethylammonium bromide | - |
Bos taurus |  |
| additional information | solublization of the enzyme by elastase or trypsin | Bos taurus |
General Stability
| General Stability | Organism |
|---|---|
| dilution, inactivates | Bos taurus |
| freezing and thawing, inactivates | Bos taurus |
| serum albumin, 2'-AMP and NaCl, stabilizes | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Elastase | little inactivation | Bos taurus | |
| heparin | - |
Bos taurus | |
| Trypsin | inactivation | Bos taurus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
1 * 30000, SDS-PAGE | Bos taurus |
| 31000 | - |
gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, by delipidation, solubilization, gel chromatography, resolubilization, ion-exchange chromatography and affinity chromatography | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
| centrum semiovale | - |
Bos taurus | - |
| cerebral white matter | - |
Bos taurus | - |
| corpus callosum | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2680 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 6 months, more than 90% of activity retained | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2',3'-cAMP + H2O | - |
Bos taurus | 2'-AMP | - |
? | |
| nucleoside 2',3'-cyclic phosphate + H2O | - |
Bos taurus | nucleoside 2'-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 27000-31000, SDS-PAGE | Bos taurus |
| monomer | 1 * 30000, SDS-PAGE | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
- |
Bos taurus |
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Release 2023.1 (January 2023)
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