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3.1.4.37: 2',3'-cyclic-nucleotide 3'-phosphodiesterase

This is an abbreviated version!
For detailed information about 2',3'-cyclic-nucleotide 3'-phosphodiesterase, go to the full flat file.

Word Map on EC 3.1.4.37

Reaction

nucleoside 2',3'-cyclic phosphate
+
H2O
=
Nucleoside 2'-phosphate

Synonyms

2',3'-cyclic AMP phosphodiesterase, 2',3'-cyclic nucleoside monophosphate phosphodiesterase, 2',3'-cyclic nucleotide 3'-phosphodiesterase, 2',3'-cyclic nucleotide 3'-phosphohydrolase, 2',3'-cyclic nucleotide phosphodiesterase, 2',3'-cyclic nucleotide phosphohydrolase, 2',3'-cyclic nucleotide-3'-phosphodiesterase, 2',3'-cyclic nucleotide-3'-phosphohydrolase, 2',3'-cyclic phosphodiesterase, 2',3'-cyclic-nucleotide 3'-phosphodiesterase type I, 2':3'-CNMP-3'-ase, 2':3'-cyclic nucleotide 3'-phosphodiesterase, 3DMA, CNP, CNP1, CNP2, CNPase, CNPase I, cyclic 2',3'-nucleotide 3'-phosphodiesterase, cyclic 2',3'-nucleotide phosphodiesterase, cyclic-CMP phosphodiesterase, DGC2, nucleoside-2':3'-cyclic-phosphate 2'-nucleotidohydrolase, PaAcpH, phosphodiesterase, cyclic 2',3'-nucleotide 3'-, PNKP, RocR, YtqI, YybT

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
                3.1.4.37 2',3'-cyclic-nucleotide 3'-phosphodiesterase

Engineering

Engineering on EC 3.1.4.37 - 2',3'-cyclic-nucleotide 3'-phosphodiesterase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D233A
-
catalytic activity is neligible
D233E
-
decrease in catalytic efficiency by factor 9
D233N
-
catalytic activity is neligible
D236A
-
increase in enzyme affinity for substrate
D236E
-
kinetics similar to wild-type
D236N
-
increase in enzyme affinity for substrate
D392A
-
decrease of both kcat and Km value
D392E
-
2fold increase in kcat value without affecting Km value
D392N
-
2fold increase in kcat value without affecting Km value
H189A
-
slight increase of Km value with 6fold decrease in kcat value
H189D
-
4fold increase in kcat value without affecting Km value
H189E
-
2fold increase in kcat value without affecting Km value
H323A
-
kinetics similar to wild-type
H323Q
-
kinetics similar to wild-type
H376D
-
60% increase in catalytic efficiency
H376N
-
twofold increase in catalytic efficiency
R237A
-
4fold increase in Km value
R237K
-
6fold increase in Km value
R237Q
-
3fold increase in Km value
D21A
tRNA nucleotidyltransferase mutant, EC 2.7.7.25, no effect on the 2',3'-cyclic phosphodiesterase activity
D23A
tRNA nucleotidyltransferase mutant, EC 2.7.7.25, no effect on the 2',3'-cyclic phosphodiesterase activity
D256A
HD domain mutant without detectable 2',3'-cyclic phosphodiesterase activity
D306A
HD domain mutant with 2',3'-cyclic phosphodiesterase activity
H225A
HD domain mutant without detectable 2',3'-cyclic phosphodiesterase activity
H305A
HD domain mutant without detectable 2',3'-cyclic phosphodiesterase activity
F235A
the mutant shows near-complete catalytic inactivation
F235L
the mutant shows near-complete catalytic inactivation
H230Q
H230Q/H309Q
H230S
H309Q
H309S
P225G
the mutant shows increased activity compared to the wild type enzyme
P296G
the mutant shows slightly increased activity compared to the wild type enzyme
R307Q
the mutant shows reduced activity compared to the wild type enzyme
T232A
the mutant shows near-complete catalytic inactivation
T311A
the mutant shows near-complete catalytic inactivation
V321A
Y168A
the mutant shows near-complete catalytic inactivation
Y168S
the mutant shows reduced activity compared to the wild type enzyme
C231A
-
CNP1 catalytic fragment mutant, kinetics
C231S
-
CNP1 catalytic fragment mutant, kinetics
C236A
-
CNP1 catalytic fragment mutant, kinetics
C236S
-
CNP1 catalytic fragment mutant, kinetics
C314A
-
CNP1 catalytic fragment mutant, kinetics
C314S
-
CNP1 catalytic fragment mutant, kinetics
C397S
-
CNP1 catalytic fragment mutant, kinetics
H230A/T232A/H309A/T311A
-
catalytically inactive
H230F
-
almost inactive CNP1 catalytic fragment mutant with 1000fold lower kcat-value and similar Km-value compared with wild-type CNP1
H230L
-
almost inactive CNP1 catalytic fragment mutant with 1000fold lower kcat-value and similar Km-value compared with wild-type CNP1
H309F
-
almost inactive CNP1 catalytic fragment mutant with 1000fold lower kcat-value and similar Km-value compared with wild-type CNP1
H309L
-
almost inactive CNP1 catalytic fragment mutant with 1000fold lower kcat-value and similar Km-value compared with wild-type CNP1
M21L
-
CNP2 mutant
S22A
-
CNP2 mutant with reduced phosphate incorporation
S23A
-
CNP2 mutant
S9A
-
CNP2 mutant with reduced phosphate incorporation
S9A/S22A
-
CNP2 double mutant without phosphate incorporation
additional information